Summary
The chromatography of monounsaturated fatty acids as their methyl esters on silver nitrate-loaded HPLC-columns has been studied. The separation of cis- and trans-isomers was easily achieved even with columns of low performance. High-performance small-particle-columns treated with silver nitrate separated a large variety of monounsaturated cis and trans positional isomers. The influence of variable silver-loads on the selectivity of the system was studied and a survey of the distribution of positional trans C18∶1 isomers in commercial margarines is given.
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References
E. Aaes-Jørgenson andG. Hølmer, Lipids4, 501 (1969).
A. J. Vergroesen (ed.), The Role of Fats in Human Nutrition, Academic Press, New York, 1975.
K. Grob, Jr.,H. P. Neukom, D. Fröhlich andR. Battaglia, J. High Res. Chromatogr./Chromatogr. Commun.1, 94 (1978).
K. E. J. Dittmar, H. Heckers andF. W. Melcher, Fette, Seifen, Anstrichmittel80, 297 (1978).
R. G. Ackman andC. A. Eaton, Fette, Seifen, Anstrichmittel80, 21 (1978).
F. Mikes, V. Schurig andE. Gil-Av, J. Chromatogr.83, 91 (1973).
R. Battaglia, Mitt. Geb. Lebensm. Hyg.68, 28 (1977).
R. Battaglia, Short-paper presentation of Euroanalysis III-Symposium in Dublin, 1978.
H.W.S. Chan andG. Levett, Chem. and Ind. (London) 1978, 578.
C. R. Scholfield, J. Am. Oil Chemists Soc.56, 510 (1979).
K. Bencze, Fette. Seifen. Anstrichmittel77, 441 (1975).
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Battaglia, R., Fröhlich, D. HPLC-separation of cis and trans monounsaturated fatty acids. Chromatographia 13, 428–431 (1980). https://doi.org/10.1007/BF02261524
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DOI: https://doi.org/10.1007/BF02261524