Summary
A reversed-phase chromatographic column suitable for the purification of chemically synthesized large oligodeoxyribonucleotides (oligo-DNA) was prepared. The specifications of this column are; the selected silica (Toyo Soda silica) with large pore size (at least 150 Å) and small particle diameter (5 μm desired) should be grafted only with monochloro alkylating reagent of long alkyl chain (sufficiently C18) so that the carbon content of the resultant packing material is 15–16%. Using this column, we could isolate the targeted large oligo-DNA (up to 50mer) in a large scale (75 μg per one cycle) from the impurities in the reaction mixture formed during the automated synthesis by the phosphite method.
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Makino, K., Matsumoto, T., Nakatsuji, Y. et al. Large scale purification of synthesized oligodeoxyribonucleotides by reversed-phase chromatography. Chromatographia 23, 909–914 (1987). https://doi.org/10.1007/BF02261470
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DOI: https://doi.org/10.1007/BF02261470