On the mechanism of solute retention in reversed-phase HPLC systems with methanol-water eluent
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In this paper we consider the possibility of gaining a better insight in the retetion mechanisms of solutes chromatographed in reversed-phase (RP) HPLC systems with the use of methanol-water binary mixtures as the mobile phase. This is done with help of the new retention model presented earlier as the relationship for the retardation coefficient (RF) of solute. The equation constants A, B and C were defined as proportional to the hypothetical retardation coefficients of the solute chromatographed separately in the three theoretically extracted moieties of the mobile phase (i.e. in pure methanol, pure water and the H-bonded associative methanol-water mixture); therefore the message imbedded in their numerical values can refer to the different and often opposite forces acting separately on the solute in a given RP-HPLC system from the side of each mobile phase moiety. An effort to decipher this message was undertaken upon the empirical results obtained for three groups of compounds, i.e., for dithia[3.3]phanes, and mono- and dihydroxyaromatics.
Key WordsColumn liquid chromatography Intermolecular interactions Retention mechanisms in RP systems
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