Determination of azidothymidine and its degradation product thymine in pharmaceutical dosage forms by HPLC and HPTLC densitometry

Summary

HPTLC densitometry and HPLC are considered for the determination of azidothymidine and its degradation product thymine in pharmaceutical dosage forms. In HPTLC the substances were separated on silica gel with fluorescence indicator in methanol-chloroform (10∶90) and methanol-chloroform (15∶85) systems. Absorbance measurement (detection of reflectance) of the separated substances was carried outin situ at 268 nm using four-level calibration (external standard, linear regression function) in the concentration range of 25–100 ng thymine/spot and using single-level calibration (external standard) at the concentration of 100 ng azidothymidine/spot. HPLC was carried out using RP-18 stationary phase and methanol+aqueous 0.03 mol/l KH2PO4 (18+82, v/v) as the mobile phase. The temperature was 50°C and the detection wavelength 266 nm. The detection limit of thymidine was 0.05%. The concentration range for azidothymidine was 0.5–1.5 mg/ml and for thymine 1–40 μg/ml (for an injection volume of 10 μl). The results were evaluated by linear regression analysis.

This is a preview of subscription content, access via your institution.

References

  1. [1]

    J. P. Horowitz, J. Chua, M. Noet, J. Org. Chem.,29, 2076 (1964).

    Google Scholar 

  2. [2]

    H. Mitsuya, K. J. Weinhold, P. A. Furman, M. H. Stclair, S. N. Lehrman, R. C. Gallo, D. Bolognesi, D. W. Barry, S. Broder, Proc. Natl. Acad. Sci. USA82, 7096 (1985).

    PubMed  Google Scholar 

  3. [3]

    W. P. Parks, E. S. Parks, J. Acquired Immune Deficiency Syndromes,1, 125 (1988).

    Google Scholar 

  4. [4]

    E. D. Bashaw, M. A. Amantea, Amer. J. Hospital Pharmacy,45, 2532 (1988).

    Google Scholar 

  5. [5]

    M. A. Skinner, T. J. Matthews, J. Acquired Immune Deficiency Syndrome1, 162 (1988).

    Google Scholar 

  6. [6]

    V. Herbert, S. Hirschman, JAMA,260, 3586 (1988).

    Google Scholar 

  7. [7]

    I. V. D. Weller, A. Mills, British Medical J.,297, 1608 (1988).

    Google Scholar 

  8. [8]

    S. E. Keller, S. J. Schleifer, J. A. Bartlett, R. L. Johnson, JA MA,260, 3586 (1988).

    Google Scholar 

  9. [9]

    H. D. Langtry, D. M. Campoli-Richards, Drugs,37, 40 (1989).

    Google Scholar 

  10. [10]

    P. A. Cload, J. Infection18, Suppl. 1, 15 (1989).

    Article  Google Scholar 

  11. [11]

    I. Williams, G. Gabriel, H. Cohen, P. Williams, R. S. Tedde, S. Machin, I. V. D. Weller, J. Infection, Suppl.1, 23 (1989).

    Article  Google Scholar 

  12. [12]

    J. M. Yeo, J. Infection18, Suppl. 1, 93 (1989).

    Article  Google Scholar 

  13. [13]

    L. E. Mathes, G. Muschik, L. Demby, P. Polas, D. W. Melini, H. J. Issaq, R. Sams, J. Chromatogr.,432, 346 (1988).

    PubMed  Google Scholar 

  14. [14]

    J. D. Unadkat, S. S. Crosby, J. P. Wang, C. C. Hertel, J. Chromatogr.,430, 420 (1988).

    PubMed  Google Scholar 

  15. [15]

    M. A. Hedaya, R. J. Sawchuk, Clin. Chem.,34, 1565 (1988).

    PubMed  Google Scholar 

  16. [16]

    S. S. Good, D. J. Reynolds, P. de Miranda, J. Chromatogr.431, 123 (1988).

    PubMed  Google Scholar 

  17. [17]

    M. R. Blum, S. H. T. Liao, S. S. Good, P. de Miranda, Amer. J. Med.,85, Suppl. 2A, 189 (1988).

    Google Scholar 

  18. [18]

    W. J. M. Underberg, U. K. Underberg-Chitoe, O. Bekers, L. Meenhorst, J. H. Beijnen, Internat. J. Pharmaceutics,50, 175 (1989).

    Article  Google Scholar 

  19. [19]

    Analytical Procedures — Azidothymidin tablets, Lachema Czechoslovakia, 1989.

Download references

Author information

Affiliations

Authors

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Tománková, H., Šabartová, J. Determination of azidothymidine and its degradation product thymine in pharmaceutical dosage forms by HPLC and HPTLC densitometry. Chromatographia 29, 44–50 (1990). https://doi.org/10.1007/BF02261138

Download citation

Key Words

  • Column liquid chromatography
  • Thin layer chromatography
  • Azidothymidine
  • Thymine