Chromatographia

, Volume 28, Issue 9–10, pp 493–496 | Cite as

The effects of buffer salt concentration on the HPLC retention of nucleic acid components

  • Guanghui Liu
Originals

Summary

In the reversed-phase high-performance liquid chromatographic studies of nucleotides, nucleosides and bases, phosphate and acetate are commonly used as buffer materials. The concentration of these buffer salts in the mobile phase exerts noticeable influence both upon the retardation and upon the separation of the nucleic acid components. This can be of certain usage in the fine adjustment of the elution order of individual components. The possible reasons for the observed data are discussed.

Key Words

Column liquid chromatography Nucleic acid components Buffer salt concentration Variation of retention 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1989

Authors and Affiliations

  • Guanghui Liu
    • 1
  1. 1.Analytical CenterThe Southwest Research Institute of Chemical IndustryNaxi, SichuanPR China

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