Ion-pair chromatography with divalent counter cations in reversed-phase systems
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The chromatographic behaviour of naphthalenedisulfonates when using divalent alkylammonium ions as counter ions in a phosphate buffered mobile phase has been studied. The retention of the disulfonates was found to be based on an ion-exchange process with the adsorbed divalent cation. The retention model is based on studies of the influence of concentration of divalent counter ions, dihydrogen phosphate and sodium, as well as pH, on the retention of mono- and divalent naphthalenesulphonates. No clear indication of specific, multipoint, interactions between the naphthalenedisulfonates and the divalent counter ions was observed.
Key WordsReversed phase ion-pair chromatography Ion-exchange Divalent cationic counter ions Naphthalenesulfonates Naphthalenedisulfonates
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