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A high-performance liquid chromatographic method for determination of flecainide in human plasma using fluorescence detection

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Summary

A high performance liquid chromatographic method for the determination of flecainide in serum has been developed. The analysis is performed on a microparticulate silica column. The eluate is monitored by fluorescence detection at an excitation wavelength of 300nm and an emission wavelength of 370nm. No sources of interference were identified and a coefficient of variation of less than 8% was observed on repeated flecainide determinations. The method has a good reproducibility, specificity and accuracy, and can be applied in therapeutic drug monitoring of flecainide in patients.

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Authors and Affiliations

  1. Department of Clinical Biochemistry, Diabetic Research Institute, University of Düsseldorf, Auf'm Hennekamp 65, D-4000, Düsseldorf 1, FRG

    S. Humfeld, F. Susanto, W. Riedel & H. Reinauer

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  1. S. Humfeld
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  2. F. Susanto
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  3. W. Riedel
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  4. H. Reinauer
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Humfeld, S., Susanto, F., Riedel, W. et al. A high-performance liquid chromatographic method for determination of flecainide in human plasma using fluorescence detection. Chromatographia 28, 303–306 (1989). https://doi.org/10.1007/BF02260780

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  • DOI: https://doi.org/10.1007/BF02260780

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