, Volume 28, Issue 5–6, pp 289–292 | Cite as

A simple highly reproducible gradient system for micro column HPLC

  • H. Bauer


A gradient delivery system for microbore and open tubular liquid chromatography is described. A gradient mixing vessel of 5–100μl, or 25–1000μl allows the generation of binary gradients with high reproducibility. The suitability of the system for short packed micro bore columns and open tubular columns is demonstrated.

Key Words

Column liquid chromatography Micro-bore and capillary columns Gradient techniques 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. [1]
    D. Ishii, K. Hibi, K. Asai, M. Nagaya, J. of Chromatogr.152, 341 (1978).Google Scholar
  2. [2]
    H. E. Schwartz, B. L. Karger, P. Kucera, Anal. Chem.55, 1752 (1983).Google Scholar
  3. [3]
    M. Krejci, K. Slais, A. Kunath, Chromatographia22, 311 (1986).Google Scholar
  4. [4]
    M. Martin, G. Guiochon, J. of Chromatogr.151, 267 (1978).Google Scholar
  5. [5]
    H. Bauer, Habilitationsschrift der Universität Tübingen, 1986.Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1989

Authors and Affiliations

  • H. Bauer
    • 1
  1. 1.Physiologisch-chemisches Institut der Universität TübingenTübingenFRG

Personalised recommendations