Summary
Migration of spots in displacement development was investigated using a planar stationary phase. The effects of the presence or absence of the additive substances in the carrier were demonstrated when these compounds also played a role in the competition for the binding sites of the stationary phase; however, they were not members of the displacement train.
In the case of displacement chromatography, compounds A+B are well separated but located very closely to each other. Using a spacer (C) in the sample, the peaks or spots of A and B are aparted by C and their virtual separation is increased.
When compounds A+B were displaced by each other as well as by the displacer, the presence of the additive compound C in the carrier (carrier-spacer) can transfer an otherwise displaced component (A) into the so-called overloaded elution region of the chromatogram. At the same time the additive compound C does not unconditionally reduce the performance of displacement chromatography.
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Kalász, H., Báthori, M. & Nagy, J. Displacement and spacer displacement chromatography. Alteration of spot profile in displacement thin-layer chromatography. Chromatographia 27, 253–256 (1989). https://doi.org/10.1007/BF02260457
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DOI: https://doi.org/10.1007/BF02260457