Skip to main content
SpringerLink
Log in

High-performance liquid chromatographic assay of ampicillin in plasma and broncho-alveolar lavage fluid, using fluorescence detection

  • Late Papers from the 17th International Symposium on Chromatography, Vienna, Austria, September 25–30, 1988
  • Published:
Chromatographia Aims and scope Submit manuscript

Summary

A high performance liquid chromatographic analysis of ampicillin in plasma and broncho-alveolar lavage fluid, using amoxicillin as internal standard, is described. The samples are deproteinated with trichloroacetic acid. For both ampicillin and amoxicillin, a fluorescent degradation product is formed in the presence of mercury(II)-chloride, after alkaline degradation and acid hydrolysis. Chromatography is done on a Spherisorb 5 ODS column using methanol-water (60∶40) with PicRB-7 (1-heptane sulphonic acid) as mobile phase and fluorescence detection (Ext 345 nm, Em 425 nm). Calibration curves for ampicillin were linear over the concentration range 1–100 μg/ml in 100 μl plasma samples and over the concentration range 20–2000 ng/ml in 0.5 ml samples of broncho-alveolar lavage fluid.

Quantitative analyses with relative standard deviations of less than 10% are achieved.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. J. Symonds, Brit. Med. J.,294, 1181–1182 (1987).

    Google Scholar 

  2. T. B. Vree, Y. A. Hekster, A. M. Baars, E. van der Kleijn, J. Chromatogr.,145, 496–501 (1978).

    Google Scholar 

  3. H. J. Rogers, I. D. Bradbrook, P. J. Morrison, R. G. Spector, D. A. Cox, L. J. Lees, J. Antimicrob. Chemother.,11, 435–445 (1983).

    Google Scholar 

  4. E. A. Ibrahim, M. E. Abdel-Hamid, M. A. Abuirjeie, A. M. Hurani, Anal. Lett.21, 423–434 (1988).

    Google Scholar 

  5. J. Haginaka, J. Wakai, J. Pharm. Pharmacol.,39, 5–8 (1987).

    Google Scholar 

  6. J. Haginaka, J. Wakai, H. Yasuda, T. Uno, K. Takahashi, T. Katagi, J. Chromatogr.,400, 101–111 (1987).

    Google Scholar 

  7. K. Miyazaki, K. Ohtani, K. Sunada, T. Arita, J. Chromatogr.,276, 478–482 (1983).

    Google Scholar 

  8. M. J. Lebelle, A. Vilim, W. L. Wilson, J. Pharm. Pharmacol.,31, 441–443 (1979).

    Google Scholar 

  9. J. Carlqvist, D. Westerlund, J. Chromatogr.,344, 285–296 (1985).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Heymans Institute of Pharmacology, University of Gent Medical school, De Pintelaan 185, B-9000, Gent, Belgium

    M. T. Rosseel & M. G. Bogaert

  2. Department of Chest Diseases, University of Gent Medical School, De Pintelaan 185, B-9000, Gent, Belgium

    Y. J. Valcke

Authors
  1. M. T. Rosseel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. G. Bogaert
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Y. J. Valcke
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rosseel, M.T., Bogaert, M.G. & Valcke, Y.J. High-performance liquid chromatographic assay of ampicillin in plasma and broncho-alveolar lavage fluid, using fluorescence detection. Chromatographia 27, 243–246 (1989). https://doi.org/10.1007/BF02260455

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02260455

Key Words

Search

Navigation