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Enhanced chemiluminescence detection of some dansyl amino acids in liquid chromatography

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Summary

The dansylated amino acids alanine, glutamic acid, methionine and norleucine were separated by reversedphase HPLC and detected via chemical excitation using the post-column, TCPO-peroxyoxalate reaction system. Enhancement of the chemiluminescence emission was achieved by including the surfactant Triton X-100 in the eluent.

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Authors and Affiliations

  1. Laboratory of Pharmaceutical Chemistry and Drug Analysis, Faculty of Pharmaceutical Sciences, State University of Ghent, Harelbekestraat 72, B-9000, Ghent, Belgium

    W. Baeyens, J. Bruggeman & B. Lin

  2. Laboratory of Instrumental Techniques, Faculty of Pharmacy, Complutense University of Madrid, 28040, Madrid, Spain

    B. Lin

Authors
  1. W. Baeyens
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  2. J. Bruggeman
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  3. B. Lin
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Baeyens, W., Bruggeman, J. & Lin, B. Enhanced chemiluminescence detection of some dansyl amino acids in liquid chromatography. Chromatographia 27, 191–193 (1989). https://doi.org/10.1007/BF02260444

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  • DOI: https://doi.org/10.1007/BF02260444

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