Chromatographia

, Volume 15, Issue 8, pp 498–504 | Cite as

A chromatographic response function for automated optimization of separations

  • W. Wegscheider
  • E. P. Lankmayr
  • K. W. Budna
Originals

Summary

A chromatographic response function is studied that is sensitive to important criteria of analytical performance: selectivity, accuracy, precision and time required to perform the separation. This function can be applied to mixtures of unknown solutes and little computational effort is needed to evaluate the numerical value of the function from the chromatogram. It shows well defined optima at elution strengths that give minimal elution times simultaneously with good separations in high-performance liquid chromatography. No prior chromatographic information characterizing the expected performance of a system is required. It is possible to use this function without a mechanistic model describing the separation process.

Key Words

Analytical performance Chromatographic response function HPLC (or LC) Optimization 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1982

Authors and Affiliations

  • W. Wegscheider
    • 1
  • E. P. Lankmayr
    • 1
  • K. W. Budna
    • 1
  1. 1.Institute for Analytical Chemistry, Micro- and RadiochemistryTechnical UniversityGrazAustria

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