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Chromatographia

, Volume 14, Issue 4, pp 249–253 | Cite as

Fluorimetric reaction detectors for high-performance liquid chromatography

Part 1. Selection of chemical reactions
  • G. Schwedt
  • E. Reh
Green Pages

Summary

Using selected steroids proposals are investigated for coupling liquid chromatographic separations with derivatization after separation for fluorimetric detection. Reverse-phase-, adsorption chromatography, systems with chemically bonded phases as well as one-step reaction with isonicotinoylhydrazide in organic solvents and a multistep reaction of ketosteroids to form dihydrolutidine derivatives in aqueous solution were combined. The effects of the optimized reaction conditions in an air-segmented mixing coil reactor and the mobile phase on fluorescence yield and detection limit are exemplified and discussed. The detection limits for corticosterone are 7 resp. 100 ng in the reactor, when connected to a HPLC-system 100 resp. 500 ng (one-step resp. multi-step reaction).

Key Words

Reaction detector HPLC Steroids Fluorimetry Coupling conditions 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1981

Authors and Affiliations

  • G. Schwedt
    • 1
  • E. Reh
    • 1
  1. 1.Fachbereich 8-Analytische ChemieUniversität-Gesamthochschule SiegenSiegen 21Federal Republic of Germany

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