Abstract
A mixture of five genomic clones spanning theα-S2 casein gene (CSN1S2) were mapped to river buffalo (Bubalus bubalis L.) and cattle (Bos taurus L.) chromosomes by fluorescencein situ hybridization (FISH) and R-banding. Clear probe hybridization signals were detected on river buffalo chromosome 7q, band 32, and the homologous cattle chromosome. These mapping data allow the indirect assignment of the entire cattle U15 syntenic group to river buffalo chromosome 7. The assignment of U15 to river buffalo chromosome 7 is discussed in the light of chromosomal nomenclature discrepancies involving the homologous cattle chromosome.
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References
Ansari HA, Pearce PD, Maher DW, Malcolm AA, Broad TE (1993) Resolving ambiguities in the karyotype of domestic sheep (Ovis aries).Chromosoma 102: 340–347.
Barendse W, Armitage SM, Kossarek LMet al. (1994) A genetic map of the bovine genome.Nature Genet 6: 227–235.
Bishop MD, Kappes SM, Keele JWet al. (1994) A genetic linkage map for cattle.Genetics 136: 619–639.
Broad TE, Burkin DJ, Cambridge LMet al. (1994) Seven loci on human chromosome 4 map onto sheep chromosome 6: a proposal to restore the original nomenclature of this sheep chromosome.Mammal Genome 5: 429–433.
Burghete I, Di Berardino D, Lioi MB, Taibi L, Matassino D (1987) Cytogenetic observations on a Robertsonian translocation in Saanen goats.Genet Sel Evol 19: 391–398.
Committee for Standardized Karyotype ofBubalus bubalis (1994) Standard karyotype of the river buffalo (Bubalus bubalis L, 2n=50) Report of the committee for the standardization of banded karyotypes of the river buffalo (Iannuzzi L, coordinator).Cytogenet Cell Genet 67: 102–113.
Di Meo GP, Iannuzzi L, Perucatti A, Ferrara L (1993) Identification of nucleolus organizer chromosomes in sheep (Ovis aries L) by sequential GBG/Ag-NOR and RBG/Ag-NOR techniques.Cytobios 75: 183–190.
Fries R, Eggen A, Womack JE (1993) The bovine genome map.Mammal Genome 4: 405–428.
Gallagher DS, Ryan AM, Liou LS, Sastry KN, Womack JE (1993) Somatic cell mapping of conglutinin (CGN1) to cattle syntenic group U29 and fluorescencein situ localization to chromosome 28.Mammal Genet 4: 716–719.
Gallagher DS, Schelling CP, Groenen MMA, Womack JE (1994) Confirmation that casein gene cluster resides on cattle chromosome 6,Mammal Genome 5: 524.
Groenen MAM, Dijkhof RJM, Verstege AJM, van der Poel JJ (1993) The complete sequence of the gene encoding bovineαS2-casein.Gene 123: 187–193.
Hayes H, Petit E, Bouniol C, Popescu P (1993) Localization of the alpha S2-casein gene (CASAS2) to the homologous cattle, sheep and goat chromosomes 4 by in situ hybridization.Cytogenet Cell Genet 64: 281–285.
Iannuzzi L, Di Meo GP (1995) Chromosomal evolution in bovids: a comparison of cattle, sheep and goat G- and R-banded chromosomes and cytogenetic divergences among cattle, goat and river buffalo sex chromosomes.Chrom Res 3: 1–9.
Iannuzzi L, Di Meo GP, Perucatti A, Ferrara L (1990) A Comparison of G- and R-banding in cattle and river buffalo prometaphase chromosomes.Caryologia 43: 283–290.
Iannuzzi L, Gallagher DS, Ryan AM, Di Meo GP, Womack JE (1993). Chromosomal localization of omega and trophoblast interferon genes in cattle and river buffalo by sequential R-banding and fluorescent in situ hybridization.Cytogenet Cell Genet 62: 224–227.
Iannuzzi L, Di Meo GP, Gallagher DS, Ryan AM, Ferrara L, Womack JE (1994) Gene mapping in river buffalo by FISH: present status and localization ofUMPS andCGN1 genes to chromosomes 1q31 and 4p16.Proceedings of the 11th European Colloquium on the Cytogenetics of Domestic Animals, The Royal Veterinary Agricultural University, Institute of Animal Science and Animal Health, Department of Animal Genetics (eds). Frederiksberg, Denmark, August 2–5, pp. 58–62.
Iannuzzi L, Di Meo GP, Perucatti A, Ferrara L (1995). G- and R-banding comparison of sheep (Ovis aries L.) chromosomes.Cytogenet Cell Genet 68: 85–90.
ISCNDA 1989 (1990). International system for cytogenetic nomenclature of domestic animals.Cytogenet Cell Genet 53: 65–79.
Neibergs HL, Gallagher DS, Georges Met al. (1993) Physical mapping of inhibin beta-A in domestic cattle.Mammal Genome 4: 328–332.
Reading Conference 1976 (1980) Proceedings of the First International Conference for the Standardisation of Banded Karyotypes of Domestic Animals.Hereditas 92: 145–162.
Threadgill DW, Womack JE (1990) Genomic analysis of the major bovine milk protein genes.Nucleic Acids Res 18: 6935–6942.
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Iannuzzi, L., Gallagher, D.S., Womack, J.E. et al. FISH mapping of theα-S2 casein gene on river buffalo and cattle chromosomes identifies a nomenclature discrepancy in the bovine karyotype. Chromosome Res 4, 159–162 (1996). https://doi.org/10.1007/BF02259710
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DOI: https://doi.org/10.1007/BF02259710