Skip to main content
SpringerLink
Log in

Separation and determination of neuraminic acids in nanogram quantities by liquid chromatography — A comparative study with different amino phases

  • Originals
  • Published:
Chromatographia Aims and scope Submit manuscript

Summary

Neuraminic acid derivatives were separated easily by liquid chromatography on amino phases and using acetonitrile/15 mM phosphate buffer, pH 5.2 as mobile phase. Detection at 200 nm allowed determination of substances in the nanomole range. All solvent systems were used isocratically at room temperature. The separation did not depend on ion-exchange but on a partition mechanism, the solutes being separated according to their polarity. Thus, it was possible to optimize the resolution by varying the stationary phase and the composition of the eluent.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. R. Schauer, Adv. Carbohydr. Chem. Biochem.40 (1980).

  2. R. Schauer, Methods Enzymol. 50 C, 64–84 (1978).

    Google Scholar 

  3. R. Schauer, A. Corfield, in: Cell Biology Monographs, Vol. 10, Sialic Acids, Chemistry, Metabolism and Function, 77–84 (ed. byR. Schauer), Springer Verlag, Wien-New York (1982).

    Google Scholar 

  4. J. F. G. Vliegenthart et al., in: Sialic Acids, 95–172 (1982).

  5. J. Haverkamp, H. van Halbeek, L. Dorland, J. F. G. Vliegenthart, R. Pfeil, R. Schauer, Eur. J. Biochem.122, 305–311 (1982).

    Google Scholar 

  6. Personal communication,R. Schauer.

  7. H. K. B. Silver, K. A. Karim, M. J. Grey, F. A. Salinas, J. Chromatogr.224, 381–388 (1981).

    Google Scholar 

  8. A. K. Shukla, R. Schauer, J. Chromatogr.244, 81–83 (1982).

    Google Scholar 

  9. A. K. Shukla, N. Scholz, E. H. Reimerdes, R. Schauer, Anal. Biochem.123, 78–82 (1982).

    Google Scholar 

  10. F. Nachtmann, H. Spitzy, R. W. Frei, Anal. Chem.48, 1576–1579 (1976).

    Google Scholar 

  11. P. Orth, H. Engelhardt, Chromatographia15, 91–96 (1982).

    Google Scholar 

  12. H.-P. Buscher, J. Casals-Stenzel, R. Schauer, Eur. J. Biochem.50, 71–82 (1974).

    Google Scholar 

  13. L. Warren, J. Biol. Chem.234, 1971 (1959).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biochemie, Universität des Saarlandes, D-6600, Saarbrücken, FRG

    H. Fiedler & H. Faillard

Authors
  1. H. Fiedler
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Faillard
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fiedler, H., Faillard, H. Separation and determination of neuraminic acids in nanogram quantities by liquid chromatography — A comparative study with different amino phases. Chromatographia 20, 231–234 (1985). https://doi.org/10.1007/BF02259693

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02259693

Key Words

Search

Navigation