Summary
An equilibrium sandwich chamber for continuous thinlayer chromatography was used to study overloaded systems of the heptane + methylene chloride-silica type. Mixtures of two or three dyes were used as the model samples. Wide starting zones were formed using a glass distributor (frontal chromatography stage), then the movement of the zones was recorded during continuous elution. The effect of sample concentration and volume on the maximum separation yield was investigated. Band compression effects are illustrated for samples dissolved in solvents having a low eluent strength. Satisfactory analogy was found to separations in preparative column chromatography. Good separation yield was obtained for frontal + elution TLC: depending on the differences in the RF values of the components, 6–16 mg samples were completely separated on 0.5×100×75 mm layers containing ca. 1 g of silica.
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References
L. R. Snyder, J. J. Kirkland, Introduction to Modern Liquid Chromatography, Wiley-Interscience, N. Y., 2nd ed. 1979, Chapter 15.
Cs. Horváth, A. Nahum, J. F. Franz, J. Chromatogr.218, 365 (1981).
H. Kalász, Cs. Horváth, J. Chromatogr.215, 295 (1981);239, 423 (1982).
J. J. DeStefano, J. J. Kirkland, Anal. Chem.47, 1103A, 1193A, (1975).
M. Verzele, E. Geeraert, J. Chromatogr.18, 559 (1980).
A. W. J. DeJong, Some Aspects of Preparative Liquid Chromatography. Thesis, Univ. of Amsterdam 1980 [see also J. Chromatogr.193, 181 (1980)].
F. Geiss, Parameter der Dünnschicht-Chromatographia, Vieweg, Braunschweig, 1972.
E. Soczewiński, W. Gotkiewicz, J. Chromatogr.118, 91 (1976).
E. Soczewiński, T. Dzido, W. Gotkiewicz, K. Gazda, J. Chromatogr.131, 408 (1977).
E. Soczewiński, J. Chromatogr.138, 443 (1977): Instruction Manual for TLC sandwich tank with a glass distributor. Polish Reagents POCh, Lublin 1981.
E. Soczewiński, T. Wawrzynowicz, Chromatographia11, 466 (1978).
E. Soczewiński, W. Maciejewicz, J. Chromatogr.176, 247 (1979).
E. Soczewiński, B. Psionka, J. Kuczmierczyk, J. Liq. Chromatogr.3, 1829 (1980).
S. Hara, J. Chromatogr.137, 41 (1977).
J. E. Paanakker, J. C. Kraak, H. Poppe, J. Chromatogr.149, 111 (1979).
T. Wawrzynowicz, E. Soczewiński, J. Chromatogr.169, 191 (1979).
E. Soczewiński, J. Jusiak, Chromatographia14, 723 (1981).
E. Soczewiński, T. Wawrzynowicz, J. Chromatogr.218, 729 (1981).
J. Lankelma, H. Poppe, J. Chromatogr.149, 587 (1978).
W. Gotkiewicz, C. E. Werkhoven-Goewie, U. A. Th. Brinkman, R. W. Frei, H. Colin, G. Guiochon, J. Chromatogr. Sci.21, 27 (1983).
T. Wawrzynowicz, E. Soczewiński, in press.
E. Tyihák, E. Mincsovics, H. Kalász, J. Nagy, J. Chromatogr.211, 45 (1981).
G. Guiochon, L. A. Beaver, M. F. Gonnord, A. M. Siouffi, M. Zakaria, J. Chromatogr.415, 255 (1983).
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Wawrzynowicz, T., Soczewiński, E. & Czapińska, K. Formation and migration of zones in overloaded preparative liquid-solid chromatography. Chromatographia 20, 223–227 (1985). https://doi.org/10.1007/BF02259691
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DOI: https://doi.org/10.1007/BF02259691