Skip to main content
SpringerLink
Log in

Improved quantitative thin-layer chromatographic method for the separation of cobalamin derivatives

  • Originals
  • Published:
Chromatographia Aims and scope Submit manuscript

Summary

A new quantitative thin-layer chromatographic method was developed for the separation and determination of cobalamin derivatives. A mixture of acetone, acetonitrile, isopropanol, diethylamine and aqueous ammonium hydroxide as the eluent and precoated Kieselgel 60 chromatoplate as the TLC plate was used. The evaluation of the separated spots was carried out by densitometry. The method can be used for the determination of cyanocobalamin in fermentatic mixture without any cleaning or concentration procedure as well as for the purity test of cyanocobalamin and hydroxocobalamin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. G. Tortolani, P. G. Ferri, J. Chromatogr.88, 430 (1974).

    Article  Google Scholar 

  2. I. M. Hais, K. Macek, Handbook of Paper Chromatography, p. 64. Akadémiai Kiadó, Budapest (Hungarian edition) (1961).

    Google Scholar 

  3. A. Saucine, L. Ionescu, M. Albu-Budai, Rev. Chim. Bucharest18, 237 (1967).

    Google Scholar 

  4. H. Vogelman, F. Wagner, J. Chromatogr.76, 359 (1973).

    Article  PubMed  Google Scholar 

  5. S. Goldstein, A. Duca, Rev. Chim. Bucharest24, 871 (1974).

    Google Scholar 

  6. G. Tortolani, V. Mantovani, J. Chromatogr.92, 201 (1974).

    Article  Google Scholar 

  7. B. Petrangeli, Boll. Chim. Farm. Milano105, 770 (1966).

    Google Scholar 

  8. B. Petrangeli, Boll. Chim. Farm. Milano106, 629 (1967).

    Google Scholar 

  9. B. Petrangeli, Boll. Chim. Farm. Milano106, 633 (1967).

    Google Scholar 

  10. British Pharmacopoeia Edition 80, p. 129, 229, University Press, Cambridge (1980).

  11. The Pharmacopoeia of Japan Ninth Edition, p. 273, Yakuji Nippo, Ltd., Tokyo (English edition) (1978).

  12. M. Govello, O. Schettino, Farmaco ed. Prat. Pavia19, 38 (1964).

    Google Scholar 

  13. T. Sasaki, J. Chromatogr.24, 452 (1966).

    Article  PubMed  Google Scholar 

  14. A. Camellini, G. Schiatti, Boll. Chim. Farm. Milano106, 463 (1967).

    PubMed  Google Scholar 

  15. R. A. Firth, H. A. O. Hill, J. M. Pratt, R. G. Thorp, Anal. Biochem.23, 429 (1968).

    Article  PubMed  Google Scholar 

  16. B. Lotti, Boll. Chim. Farm. Milano114, 416 (1975).

    PubMed  Google Scholar 

  17. C. J. Blake, Res. Assoc. Sci. Techn. Surv.95, 46 (1977).

    Google Scholar 

  18. F. Pellerin, J. F. Letavernier, N. Chanon, Ann. Pharm. Fr.35, 413 (1977).

    PubMed  Google Scholar 

  19. G. Szepesi, M. Gazdag. (in preparation).

Download references

Author information

Authors and Affiliations

  1. Chemical Works of Gedeon Richter, Ltd., Post Office Box 27, 1475, Budapest 10, Hungary

    G. Szepesi & J. Molnár

Authors
  1. G. Szepesi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Molnár
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Szepesi, G., Molnár, J. Improved quantitative thin-layer chromatographic method for the separation of cobalamin derivatives. Chromatographia 14, 709–711 (1981). https://doi.org/10.1007/BF02259461

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02259461

Key Words

Search

Navigation