, Volume 25, Issue 11, pp 983–988 | Cite as

Unusual signal enhancement in reversed-phase chromatography of polystyrene monitored by UV detector

  • G. Glöckner
  • S. Schmutzler
  • H. Engelhardt
  • R. Schultz


Gradient elution of polystyrene standards on reversed phase C18 columns by methanol/tetrahydrofuran or methanol/dichloromethane mixtures yielded a strange effect of the molecular weight of the sample on the specific peak are (mAUs per μg sample injected). The effect did not occur when pure dichloromethane was used as an eluent. Further to this, dependence of specific peak area on flow rate was observed in gradient elution with methanol/tetrahydrofuran mixtures. It was found that these effects were due to polymer elution at the verge of precipitation. Depending on the dwell time of the sample in the column, opalescence occurred that added to the UV signal used for monitoring the elution.

Key Words

Column liquid chromatography UV detection Solubility Polystyrene Sample precipitation Gradient elution 


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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1988

Authors and Affiliations

  • G. Glöckner
    • 1
  • S. Schmutzler
    • 1
  • H. Engelhardt
    • 2
  • R. Schultz
    • 2
  1. 1.Sektion ChemieTechnische Universität DresdenDresdenGDR
  2. 2.Angewandte Physikalische ChemieUniversität des SaarlandesSaarbrückenFRG

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