Summary
The contributions of various functional groups to the retention of non-ionogenic compounds such as cardiac glycosides and steroid hormones was studied in reversed-phase liquid chromatography. The dependence of these contributions on the surface chemistry of the adsorbent, the mobile phase composition and column temperature was also investigated. From the dependence of capacity factor k′ of glycosiedes on the column temperature at various mobile phase compositions one can calculate the changes in the enthalpy of adsorption from solution at small coverage. The correlation between the biological activity of the cardiac glycosides and their retention in reversed-phase liquid chromatography was determined. It was demonstrated that this correlation can be used for the evaluation of the biological activity as a function of the contribution of different functional groups to the retention.
Similar content being viewed by others
References
A. V. Kiselev, “Fizicheskaya Khimiya. Sovremennye Problemy” (Modern Problems of Physical Chemistry), Vol. 2, Khimiya Publisher, Moscow, 1982.
V. Ya. Davydov, in Chromatography-84, L. S. Ettre and H. Kalász, eds., Akadémiai Kiadé, Budapest, 1986, p. 351.
Y. Ya. Davydov, J. Chromatogr.,365, 123 (1986).
J. K. Seydel, K. J. Schaper, Chemische Struktur und Biologische Aktivität von Wirkstoffen, Verlag Chemie, Weinheim, 1979.
T. Braumann, J. Chromatogr.,373, 191 (1986).
A. P. Arzamatzev, Y. Ya. Davydov, M. E. Gonzales, A. V. Kiselev, R. A. Rodionova, Khim. Farm. Zh., No. 4, 494 (1983).
V. Ya. Davydov, M. E. Gonzalez, A. V. Kiselev, J. Chromatogr.,248, 49 (1982).
K. Valkó, J. Liquid Chromatogr.,7, 1405 (1984).
N. N. Avgul, A. V. Kiselev, Physical Adsorption of Gases and Vapours on Graphitized Carbon Black, Col. Chemistry and Physics of Carbon Ed.P. L. Walker, Dekker, New York 1970.
K. Valkó, T. Friedmann, J. Báti, A. Nagykáldi, J. Liquid Chromatogr.,7, 2073 (1984).
I. F. Makarevitch, E. P. Kemertelidze, S. G. Kislitchenko, V. V. Zatuga, V. A. Reznitchenko, D. G. Kolesnikov, I. P. Kovalev, “Kardenolidy i Bufadienolidy Metody ustanovleniya stroeniya i spravochnye dannye” (Cardenolids and Bufadienolids, Methods of Structure Definition and Reference Data), Metzniereba, Tbilisi, 1975.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Davydov, V.Y., Filatova, G.N., Smolkova-Keulemansova, E. et al. Evaluation of the retention of cardiac glycosides and steroid hormones in reversed-phase liquid chromatography and their biological activity according to the contribution of hydrophilic and hydrophobic groups to retention. Chromatographia 25, 1059–1066 (1988). https://doi.org/10.1007/BF02259385
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02259385