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Evaluation of the retention of cardiac glycosides and steroid hormones in reversed-phase liquid chromatography and their biological activity according to the contribution of hydrophilic and hydrophobic groups to retention

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Summary

The contributions of various functional groups to the retention of non-ionogenic compounds such as cardiac glycosides and steroid hormones was studied in reversed-phase liquid chromatography. The dependence of these contributions on the surface chemistry of the adsorbent, the mobile phase composition and column temperature was also investigated. From the dependence of capacity factor k′ of glycosiedes on the column temperature at various mobile phase compositions one can calculate the changes in the enthalpy of adsorption from solution at small coverage. The correlation between the biological activity of the cardiac glycosides and their retention in reversed-phase liquid chromatography was determined. It was demonstrated that this correlation can be used for the evaluation of the biological activity as a function of the contribution of different functional groups to the retention.

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References

  1. A. V. Kiselev, “Fizicheskaya Khimiya. Sovremennye Problemy” (Modern Problems of Physical Chemistry), Vol. 2, Khimiya Publisher, Moscow, 1982.

    Google Scholar 

  2. V. Ya. Davydov, in Chromatography-84, L. S. Ettre and H. Kalász, eds., Akadémiai Kiadé, Budapest, 1986, p. 351.

    Google Scholar 

  3. Y. Ya. Davydov, J. Chromatogr.,365, 123 (1986).

    Article  PubMed  Google Scholar 

  4. J. K. Seydel, K. J. Schaper, Chemische Struktur und Biologische Aktivität von Wirkstoffen, Verlag Chemie, Weinheim, 1979.

    Google Scholar 

  5. T. Braumann, J. Chromatogr.,373, 191 (1986).

    Article  PubMed  Google Scholar 

  6. A. P. Arzamatzev, Y. Ya. Davydov, M. E. Gonzales, A. V. Kiselev, R. A. Rodionova, Khim. Farm. Zh., No. 4, 494 (1983).

    Google Scholar 

  7. V. Ya. Davydov, M. E. Gonzalez, A. V. Kiselev, J. Chromatogr.,248, 49 (1982).

    Article  PubMed  Google Scholar 

  8. K. Valkó, J. Liquid Chromatogr.,7, 1405 (1984).

    Google Scholar 

  9. N. N. Avgul, A. V. Kiselev, Physical Adsorption of Gases and Vapours on Graphitized Carbon Black, Col. Chemistry and Physics of Carbon Ed.P. L. Walker, Dekker, New York 1970.

    Google Scholar 

  10. K. Valkó, T. Friedmann, J. Báti, A. Nagykáldi, J. Liquid Chromatogr.,7, 2073 (1984).

    Google Scholar 

  11. I. F. Makarevitch, E. P. Kemertelidze, S. G. Kislitchenko, V. V. Zatuga, V. A. Reznitchenko, D. G. Kolesnikov, I. P. Kovalev, “Kardenolidy i Bufadienolidy Metody ustanovleniya stroeniya i spravochnye dannye” (Cardenolids and Bufadienolids, Methods of Structure Definition and Reference Data), Metzniereba, Tbilisi, 1975.

    Google Scholar 

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Davydov, V.Y., Filatova, G.N., Smolkova-Keulemansova, E. et al. Evaluation of the retention of cardiac glycosides and steroid hormones in reversed-phase liquid chromatography and their biological activity according to the contribution of hydrophilic and hydrophobic groups to retention. Chromatographia 25, 1059–1066 (1988). https://doi.org/10.1007/BF02259385

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  • DOI: https://doi.org/10.1007/BF02259385

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