, Volume 25, Issue 12, pp 1054–1058 | Cite as

Application of HPLC to characterization and control of individual acids in apple extracts and ciders

  • D. Blanco Gomis
  • M. J. Morán Gutiérrez
  • M. D. Gutiérrez Alvarez
  • J. J. Mangas Alonso


Three HPLC methods have been tested in order to compare their separation of apple and cider organic acids. Optimum results for simultaneous determination of malic, citric, quinic, succinic, lactic and shikimic acids have been obtained using an RP-18 column with phosphate buffer as the mobile phase.

This method was applied to organic acids monitoring during the ripening of apples and throughout the fermentation process. After filtration and degasification the organic acids in the sample were separated, and quantified by using a diode array detector.

Recovery studies show good results for all solutes (88%–106%). The coefficients of variation show excellent precision (5%).

Key Words

Column Liquid Chromatography Organic acids Apple extracts 


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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1988

Authors and Affiliations

  • D. Blanco Gomis
    • 1
  • M. J. Morán Gutiérrez
    • 1
  • M. D. Gutiérrez Alvarez
    • 1
  • J. J. Mangas Alonso
    • 2
  1. 1.Departamento de Quimica Fisica y Analitica, Facultad de QuimicaUniversidad de OviedoSpain
  2. 2.Centro de Experimentación AgrariaConsejeria de Agricultura y Pesca del Principado de AsturiasVillaviciosaSpain

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