, Volume 17, Issue 12, pp 675–678 | Cite as

A comparison of eight commercial reverse-phase (ODS) columns for the separation of hydroxylated derivatives of 7,12-dimethylbenz(a)anthracene

  • B. Shaikh
  • J. E. Tomaszewski


This paper presents an evaluation of a number of commercially available octadecylsilane columns. The separation of four 7,12-dimethylbenz(a)anthracene (DMBA) derivatives namelycis- andtrans-5,6-dihydroxy DMBA,trans-8,9-dihydro-8,9-dihydroxy DMBA, and 7,12-dihydroxymethyl benz(a)anthracene (BA) was compared under identical conditions comprising of a concave gradient (No. 4) from 20 to 70% acetonitrile/water for 75 minutes at flow rate of 1ml/min. A direct correlation between percent carbon loading and retention behavior was observed for bonded phases prepared from silica particles with similar surface areas. Other column performance parameters, such as capacity factors, separation ratios and resolution between peaks for the solutes examined were also calculated and are discussed.

Key Words

HPLC Reversed-phase columns 5 and 10μm particles Dimethyl and hydroxylated derivatives of dimethylbenz(a)anthracene 


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  1. [1]
    H. Colin, G. Guiochon, J. Chromatogr.141, 289 (1977).CrossRefGoogle Scholar
  2. [2]
    R. P. W. Scott, P. Kucera, J. Chromatogr.142, 213 (1977).CrossRefGoogle Scholar
  3. [3]
    H. Colin, G. Guiochon, J. Chromatogr.158, 183 (1978).Google Scholar
  4. [4]
    E. J. Kikta, E. Grushka, Anal. Chem.48, 1098 (1976).CrossRefGoogle Scholar
  5. [5]
    K. Ogan, E. Katz, J. Chromatogr.188, 115 (1980).CrossRefGoogle Scholar
  6. [6]
    M. C. Hennion, C. Picard, M. Caude, J. Chromatogr.,166, 21 (1978).CrossRefGoogle Scholar
  7. [7]
    I. Halasz, I. Sebestian, Angew. Chem.8, 453 (1969).CrossRefGoogle Scholar
  8. [8]
    K. K. Unger, N. Becker, P. Roumeliotis, J. Chromatogr.125, 115 (1976).CrossRefGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1983

Authors and Affiliations

  • B. Shaikh
    • 1
  • J. E. Tomaszewski
    • 1
  1. 1.Chemical Carcinogenesis Program, NCIFrederick Cancer Research CenterFrederickU.S.A.

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