Use of a stationary phase with a silica-bonded electrically neutral non-macrocyclic ligand for HPLC separations of steroids
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The use of an immobilized 3,6-dioxaoctanedioic amide substrate as stationary phase is described. This stationary phase is found to be more lipophilic than the usual amino phases, but less so than C8-alkyl and cyano phases. Novel selectivities for neutral species such as steroids are demonstrated. A strong preference for equilenin is observed. Successful separations of steroid mixtures are shown.
Key WordsLiquid chromatography-reversed phase Chemically-bonded, non-macrocyclic ligands Immobilized ionophores Steroids
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