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Chromatographia

, Volume 17, Issue 12, pp 669–672 | Cite as

Use of a stationary phase with a silica-bonded electrically neutral non-macrocyclic ligand for HPLC separations of steroids

  • C. H. Loesch
  • W. Simon
Originals

Summary

The use of an immobilized 3,6-dioxaoctanedioic amide substrate as stationary phase is described. This stationary phase is found to be more lipophilic than the usual amino phases, but less so than C8-alkyl and cyano phases. Novel selectivities for neutral species such as steroids are demonstrated. A strong preference for equilenin is observed. Successful separations of steroid mixtures are shown.

Key Words

Liquid chromatography-reversed phase Chemically-bonded, non-macrocyclic ligands Immobilized ionophores Steroids 

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Copyright information

© Friedr. Vieweg & Sohn Verlagsgesellschaft mbH 1983

Authors and Affiliations

  • C. H. Loesch
    • 1
  • W. Simon
    • 1
  1. 1.Department of Organic ChemistrySwiss Federal Institute of Technology (ETH)ZürichSwitzerland

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