Summary
A new type of chromogenic reagent for labelling the amino groups of peptides or amino acids, 5-substituted 2,4-dinitrofluorobenzenes, is presented. These reagents are characterized by a polarity determined by the function included in the substituent in the 5-position and by the nature of this substituent. Preparation and chromatographic behaviour of these reagents, of eventual by-products of reactions and of some N-(2,4-dinitro-5-diethylaminophenyl)-peptides or amino acids are described.
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Proportions in solvent mixtures are v/v except otherwise indicated. Abbreviations: lit=literature, TLC=thin-layer chromatography, TLE=TL electrophoresis, see also: Figs. 2, 3, 5.
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Munier, R.L., Drapier, A.M., Meunier, S. et al. New chromogenic reagents for labelling amino groups in peptides. Chromatographic and preparatives aspects. Chromatographia 14, 289–295 (1981). https://doi.org/10.1007/BF02259268
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DOI: https://doi.org/10.1007/BF02259268