Summary
During GLC of naturally occurring mixtures of phthalides on packed columns an artefact was sometimes detected that originated from Z-ligustilide. Active sites of Chromosorb W were responsible for the formation of the artefact, that was assumed to be E-ligustilide as GC-MS revealed that its mass spectrum was identical with that of Z-ligustilide. No isomerization was observed when freshly coated Chromosorb W was used or the support was deactivated by benzoyl chloride or melamine. The identity of the artefact was confirmed by UV irradiation experiments.
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References
M. J. M. Gijbels, J. J. C. Scheffer, A. Baerheim Svendsen, Chromatographia14, 452 (1981).
M. J. M. Gijbels, J. J. C. Scheffer, A. Baerheim Svendsen, Planta Med.44, 207 (1982).
H. V. Street, W. Vycudilik, G. Machata, J. Chromatogr.168, 117 (1979).
T. N. Gvozdovich, A. V. Kiselev, Ya. I. Yashin, J. Chromatogr.116, 63 (1976).
J. Rigaudy, P. Derible, Bull. Soc. Chim. France 3047 (1965).
M. J. M. Gijbels, J. J. C. Scheffer, A. Baerheim Svendsen, Planta Med. Suppl.41 (1980).
U. E. Matter, C. Pascual, E. Pretsch, A. Pross, W. Simon, S. Sternhell, Tetrahedron25, 691 (1969).
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Gijbels, M.J.M., Scheffer, J.J.C. & Baerheim Svendsen, A. Occurrence and prevention of isomerization of ligustilide during gas-liquid chromatography on packed columns. Chromatographia 15, 358–360 (1982). https://doi.org/10.1007/BF02259218
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DOI: https://doi.org/10.1007/BF02259218