Summary
The use of high-performance liquid chromatography in kinetics and metabolism studies is demonstrated.
The following kinetic parameters are ascertained after single oral and intravenous administration of a drug: absorption by areal comparison, elimination constant in the β-phase, and clearance.
Concentration levels in serum are measured after repeated oral administration on several days, with pharmacokinetic model fit to estimate limit concentrations in the steady state. Species-dependencies in metabolism are investigated in 24-h urines.
The necessary characterization of drug and metabolite peaks is obtained by in-series connection of several detectors.
The relative bioavailability of a controlled release form is determined by measuring drug concentrations in the blood.
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References
R. Beckmann, Arch. int. Pharmacodyn.160, 161 (1966).
J. G. Wagner, J. Pharmacokinet. Biopharm.1, 165 (1973).
J. S. Wold andL. J. Fischer, J. Pharmacol.183, 188 (1972).
K. Klemm, W. Krastinat andU. Krüger, Arzneim.-Forsch./Drug Res.29 (I), 1, 1 (1979).
G. Ludwig, H. Vergin andK. Zech, Arzneim.-Forsch./Drug Res.27 (II), 11, 2077 (1977).
K. Zech andG. Ludwig, Chromatographia12, 638 (1976).
C. M. Metzler, G. L. Elfring andA. J. McEwen, A User's Manual for NONLIN and Associated Programs, Upjohn Co., Kalamazoo, Mich., April 20, 1974.
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Zech, K., Arnold, P., Liechti, H. et al. HPLC in pharmacokinetics and metabolism studies. Chromatographia 12, 354–358 (1979). https://doi.org/10.1007/BF02259153
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DOI: https://doi.org/10.1007/BF02259153