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Ion-pair-reversed-phase high-performance liquid chromatographic determination of porphyrins from red blood cells

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Summary

An extraction and ion-pair reversed-phase HPLC procedure is described for the separation and determination of protoporphyrin and its zinc chelate from red blood cells. The recoveries were greater than 90% with coefficients of variation between 8 and 10%. A linear response curve was obtained from 800 to 4000 μg/l erythrocytes for the porphyrins.

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Authors and Affiliations

  1. Institut für Klinische Chemie am Klinikum Grosshadern der Universität München, P.O.B. 701260, D-8000, München 70, Federal Republic of Germany

    H. D. Meyer, K. Jacob & W. Vogt

Authors
  1. H. D. Meyer
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  2. K. Jacob
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  3. W. Vogt
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Meyer, H.D., Jacob, K. & Vogt, W. Ion-pair-reversed-phase high-performance liquid chromatographic determination of porphyrins from red blood cells. Chromatographia 16, 190–191 (1982). https://doi.org/10.1007/BF02258897

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  • DOI: https://doi.org/10.1007/BF02258897

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