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An improved optimization procedure for the selection of mixed mobile phases in reversed phase liquid chromatography

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Summary

The stepwise procedure described previously [1] for the selection of optimal mobile phase compositions in reversed phase liquid chromatography (RPLC) is improved in two ways. The optimization criterion is modified to account for variations in analysis time. The iterative approach to the optimal solvent composition makes a more efficient use of previous data. An example illustrates the resulting gain in speed and accuracy.

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Abbreviations

A:

coefficient of the quadratic term for ln k′ vs κ

ACN:

acetonitrile

CRF:

chromatographic response function

DPA:

diphenylamines

k′:

capacity factor

MeOH:

methanol

N:

number of theoretical plates

n:

total number of solutes

Pi :

peak separation number

r:

relative resolution product

r* :

relative resolution product normalized to a given starting point

Rs:

resolution factor

t:

time(s)

THF:

tetrahydrofuran

x:

fraction of two binary compositions in a ternary mixture

x′:

shifted composition with regard to x

δ:

largest accepted uncertainty in predicted in k′ values

Δx:

distance between two measured ternary compositions

Λ:

forbidden area for measurements

Π:

product of peak separation numbers

ΠRs:

product of resolution factors

Σ0:

summation sign

References

  1. P. J. Schoenmakers, A. C. J. H. Drouen, H. A. H. Billiet, L. de Galan. Chromatographia15, (1982).

  2. P. J. Schoenmakers, H. A. H. Billiet, L. de Galan, J. Chromatogr.205, 13 (1981).

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  3. S. L. Morgan, S. N. Deming, J. Chromatogr,112, 267 (1975).

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  4. S. L. Morgan, S. N. Deming, Sep. Purif. Methods5, 333 (1976).

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  5. R. Kaiser, Gas chromatography, Geest & Portig, Leipzig, 1960, p. 33.

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  6. P. J. Schoenmakers, H. A. H. Billiet, L. de Galan, J. Chromatogr.218, 261 (1981).

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Authors and Affiliations

  1. Laboratorium voor Analytische Scheikunde, Technische Hogeschool Delft, Jaffalaan 9, 2628 BX, Delft, The Netherlands

    A. C. J. H. Drouen, H. A. H. Billiet, P. J. Schoenmakers & L. de Galan

Authors
  1. A. C. J. H. Drouen
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  2. H. A. H. Billiet
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  3. P. J. Schoenmakers
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  4. L. de Galan
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Drouen, A.C.J.H., Billiet, H.A.H., Schoenmakers, P.J. et al. An improved optimization procedure for the selection of mixed mobile phases in reversed phase liquid chromatography. Chromatographia 16, 48–52 (1982). https://doi.org/10.1007/BF02258868

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  • DOI: https://doi.org/10.1007/BF02258868

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