Skip to main content

Binding of99mTc-labelled polyclonal human immunoglobulin to bacteria as a mechanism for scintigraphic detection of infection

European Journal of Nuclear Medicine volume 18, pages 396–400 (1991)Cite this article

Abstract

The aim of the present study was to determine whether99mTc-labelled polyclonal human immunoglobulin (99mTc-HIG) binds to bacteria in vitro as well as in vivo. In vitro, the binding of99mTc-HIG to various gram-positive and gram-negative bacteria was determined. In vivo, mice were infected withStaphylococcus aureus Cowan I (protein A rich) orS. aureus EMS (protein A deficient) in a thigh muscle and then99mTc-HIG or99mTc-labelled human serum albumin (99mTc-HSA) was administered; scintigrams were made 1, 4, and 18 h later. In vitro binding of99mTc-HIG to bacteria was higher for gram-positive than for gram-negative forms. A positive correlation was found between the protein A content and the degree of binding toS. aureus. This was also found in vivo. The accumulation of99mTc-HIG at the site of infection was significantly (P < 0.01) higher than that of99mTc-HSA, for both strains ofS. aureus. It is concluded that vascular permeability cannot fully explain the accumulation of99mTc-HIG at the site of infection and that binding of99mTc-HIG to bacteria plays a role in this respect.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

References

  • Blok D, van Ogtrop M, Arndt JW, Camps JAJ, Feitsma RIJ, Goedemans W, Pauwels EKJ (1990) Detection of inflammatory lesions with radiolabefed immunoglobulins. Eur J Nucl Med 16:303–305

    Article  PubMed  Google Scholar 

  • Buscombe JR, Lui D, Ensing G, de Jong R, Ell PJ (1990)99mTc-human immunoglobulin (HIG) — first results of a new agent for the localization of infection and inflammation. Eur J Nucl Med 16:649–655

    Article  PubMed  Google Scholar 

  • Calame W, Feitsma RIJ, Ensing GJ, Goedemans WT, Camps JAJ, van Furth R, Pauwels EKJ (1991) Detection of a local staphylococcal infection in mice with99mTc-labeled polyclonal human immunoglobulin. J Nucl Med 32:468–474

    PubMed  Google Scholar 

  • Ey PL, Prowse SJ, Jenkin CR (1978) Isolation of pure IgGl, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose. Immunochemistry 15:429–136

    PubMed  Google Scholar 

  • Fischman AJ, Rubin RH, Khaw BA, Callahan RJ, Wilkinson R, Keech F, Nedelman M, Dragotakes S, Kramer PB, LaMuraglia GM (1988a) Detection of acute inflammation with111In-labeled nonspecific polyclonal IgG. Semin Nucl Med 18:335–344

    Google Scholar 

  • Fischman AJ, Wilkinson R, Khaw BA, Callahan RJ, Ahmad M, Locke E, Rossif ND, Strauss HW, Rubin RH (1988b) Imaging of localized bacterial infections with radiolabeled non-specific antibody fragments. J Nucl Med 29 [Suppl]:610 (abstr)

    Google Scholar 

  • Fischman AJ, Rubin RH, White JA, Locke E, Wilkinson RA, Nedelman M, Callahan RJ, Khaw BA, Strauss HW (1990) Localization of Fc and Fab fragments of nonspecific polyclonal IgG at focal sites of inflammation. J Nucl Med 31:1199–1205

    PubMed  Google Scholar 

  • Forsgren A (1969) Protein A fromStaphylococcus aureus. Production of protein A by bacterial and L forms ofS. aureus. Acta Pathol Microbiol Scand 75:481–490

    PubMed  Google Scholar 

  • Forsgren A, Sjöquist J (1966) Protein A fromS. aureus. I. Pseudoimmune reactions with human gammaglobulin. J Immunol 97:822–827

    PubMed  Google Scholar 

  • Goedemans WT, Panek KJ (1989) A new, simple method for labelling of proteins with99mTc. J Nucl Med All Sci 33:286

    Google Scholar 

  • Jasin HE (1988) Oxidative cross-linking of immune complexes by human polymorphonuclear leukocytes. J Clin Invest 81:6–15

    PubMed  Google Scholar 

  • Lubbe Van der PAHM, Arndt JW, Calame W, Ferreira TC, Pauwels EKJ, Breedveld FC (1991) Measurement of synovial inflammation in rheumatoid arthritis with technetium-99m labelled human polyclonal immunoglobulin G. Eur J Nucl Med 18:119–123

    Article  PubMed  Google Scholar 

  • Morrel EM, Tompkins RG, Fischman AJ, Wilkinson RA, Burke JF, Rubin RH, Strauss HW, Yarmush ML (1989) Autoradiographic method for quantitation of radiolabeled proteins in tissues using indium-111. J Nucl Med 30:1538–1545

    PubMed  Google Scholar 

  • Nardella FA, Teller DC, Barber CV, Mannik M (1985) IgG rheumatoid factors and staphylococcal protein A bind to a common molecular site on IgG. J Exp Med 162:1811–1824

    Article  PubMed  Google Scholar 

  • Oppliger IR, Nardella FA, Stone GC, Mannik M (1987) Human rheumatoid factors bear the internal image of the Fc binding region of staphylococcal protein A. J Exp Med 166:702–710

    Article  PubMed  Google Scholar 

  • Oyen WJG, Claessens RAMJ, van Horn JR, van der Meer JWM, Corstens FHM (1990) Scintigraphic detection of bone and joint infections with indium-111-labeled nonspecific polyclonal human immunoglobulin G. J Nucl Med 31:403–412

    PubMed  Google Scholar 

  • Rubin RH, Young LS, Hansen WP, Nedelman M, Wilkinson R, Nelles MJ, Callahan R, Khaw BA, Strauss HW (1988) Specific and nonspecific imaging of localized Fisher immunotype 1Pseudomonas aeruginosa infection with radiolabeled monoclonal antibody. J Nucl Med 29:651–656

    PubMed  Google Scholar 

  • Rubin RH, Fischman AJ, Needleman M, Wilkinson R, Callahan RJ, Khaw BA, Hansen WP, Kramer PB, Strauss HW (1989a) Radiolabeled, nonspecific, polyclonal human immunoglobulin in the detection of focal inflammation by scintigraphy: comparison with gallium-67 citrate and technetium-99m-labeled albumin. J Nucl Med 30:385–389

    PubMed  Google Scholar 

  • Rubin RH, Fischman AJ, Callahan RJ, Khaw BA, Keech FK, Ahmad M, Wilkinson R, Strauss HW (1989b)111In-labeled nonspecific immunoglobulin scanning in the detection of focal infection. N Engl J Med 321:935–940

    PubMed  Google Scholar 

  • Saptogino A, Becker W, Wolf F (1990)99mTc-labelled polyclonal human immunoglobulin for localization of inflammatory sites - early in vitro results. Nucl Med 29:54–58

    Google Scholar 

  • Siegel S (1956) Nonparametric statistics for the behavioral sciences. McGraw-Hill, Tokyo, pp 116–127

    Google Scholar 

  • Thakur ML, DeFulvio J, Park CH, Epstein A, McAfee J (1990) Tc-99m HSA for imaging inflammatory foci. Eur J Nucl Med 16:307 (abstr)

    Article  PubMed  Google Scholar 

Download references

Author information

Author notes

  1. Wim Calame

    Present address: Department of Cell Biology, Division of Electron Microscopy, Free University, Faculty of Medicine, P.O. Box 7161, NL-1007, MC Amsterdam, The Netherlands

Affiliations

  1. Department of Infectious Diseases, University Hospital, Building 1, C4-Q, P.O. Box 9600, NL-2300, RC Leiden

    Wim Calame & Ralph van Furth

  2. Division of Nuclear Medicine, University Hospital, Building 1, C4-Q, P.O. Box 9600, NL-2300, RC Leiden

    Wim Calame, Hans I.J. Feitsma, Jan-Willem Arndt & Ernest K.J. Pauwels

  3. Mallinckrodt Diagnostica, Petten, The Netherlands

    Geert J. Ensing

Authors
  1. Wim Calame
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hans I.J. Feitsma
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Geert J. Ensing
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jan-Willem Arndt
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ralph van Furth
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Ernest K.J. Pauwels
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Calame, W., Feitsma, H.I., Ensing, G.J. et al. Binding of99mTc-labelled polyclonal human immunoglobulin to bacteria as a mechanism for scintigraphic detection of infection. Eur J Nucl Med 18, 396–400 (1991). https://doi.org/10.1007/BF02258430

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02258430

Key words

  • Scintigraphic detection of infection
  • Immunoglobulin
  • Protein A
  • Staphylococcus aureus