Journal of Biomedical Science

, Volume 8, Issue 4, pp 365–374 | Cite as

Lamp-1 is upregulated in human glioblastoma cell lines induced to undergo apoptosis

  • Jeff W. Chen
  • Narayanamma Madamanchi
  • Nageswara R. Madamanchi
  • Todd T. Trier
  • Michael J. Keherly
Original Paper

Abstract

Lysosome-associated membrane protein (LAMP)-1, one of the major protein components of the lysosomal membrane, is upregulated in the human glioblastoma cell lines, U-373 MG and LN-Z308, which undergo cisplatin-induced apoptosis. These human brain tumor cell lines demonstrated apoptosis in response to cisplatin/nifedipine treatment. Both cell lines demonstrated an apoptotic response by more than one criterion. Apoptosis was demonstrated by DNA fragmentation techniques such as DNA laddering, ApopTag® in situ labeling, and an ELISA-based method of detecting liberated oligosomes. These cells also had characteristic morphologic changes and upregulation of bax consistent with apoptosis. LAMP-1 expression at the protein and mRNA level was examined and found to increase with cisplatin/nifedipine treatment. LAMP-1 expression was examined using indirect immunofluorescent staining, Northern blot analysis and Western blot analysis. The finding of an augmentation of LAMP-1 in these cells induced to die is enigmatic. These findings raise the possibility of LAMP-1 involvement in the apoptotic process.

Key Words

Apoptosis Brain tumor Cisplatin LAMP Lysosome 

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Copyright information

© National Science Council 2001

Authors and Affiliations

  • Jeff W. Chen
    • 1
    • 2
  • Narayanamma Madamanchi
    • 1
  • Nageswara R. Madamanchi
    • 1
  • Todd T. Trier
    • 1
  • Michael J. Keherly
    • 1
  1. 1.Division of Neurological Surgery, Department of SurgeryUniversity of Texas Medical Branch at GalvestonUSA
  2. 2.Department of Neurological SurgeryLegacy Emanuel HospitalPortlandUSA

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