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Journal of Biomedical Science

, Volume 5, Issue 2, pp 101–110 | Cite as

Protein-tyrosine kinase and protein-serine/threonine kinase expression in human gastric cancer cell lines

  • Jyh-Shi Lin
  • Chi-Wei Lu
  • Chang-Jen Huang
  • Peng-Fyn Wu
  • Daniel Robinson
  • Hsing-Jien Kung
  • Chin-Wen Chi
  • Chew-Wun Wu
  • Wen-Kang Yang
  • Jacqueline J. K. Whang-Peng
  • Wen-chang Lin
Original Paper

Abstract

Protein kinases play key roles in cellular functions. They are involved in many cellular functions including; signal transduction, cell cycle regulation, cell division, and cell differentiation. Alterations of protein kinase by gene amplification, mutation or viral factors often induce tumor formation and tumor progression toward malignancy. The identification and cloning of kinase genes can provide a better understanding of the mechanisms of tumorigenesis as well as diagnostic tools for tumor staging. In this study, we have used degenerated polymerase-chain-reaction primers according to the consensus catalytic domain motifs to amplify protein kinase genes (protein-tyrosine kinase, PTK, and protein-serine/threonine kinase, PSK) from human stomach cancer cells. Following amplification, the protein kinase molecules expressed in the gastric cancer cells were cloned into plasmid vectors for cloning and sequencing. Sequence analysis of polymerase-chain-reaction products resulted in the identification of 25 protein kinases, including two novel ones. Expression of several relevant PTK/PSK genes in gastric cancer cells and tissues was further substantiated by RT-PCR using gene-specific primers. The identification of protein kinases expressed or activated in the gastric cancer cells provide the framework to understand the oncogenic process of stomach cancer.

Key Words

Protein tyrosine kinase Protein serine/threonine kinase RT-PCR Gastric cancer 

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References

  1. 1.
    Alves F, Vogel W, Mossie K, Millauer B, Hofler H, Ullrich A. Distinct structural characteristics of discoidin I subfamily receptor tyrosine kinases and complementary expression in human cancer. Oncogene 10:609–618;1995.PubMedGoogle Scholar
  2. 2.
    Cantley LC, Auger KR, Carpenter C, Duckworth B, Graziani A, Kapeller R, Soltoff S. Oncogenes and signal transduction. Cell 64:281–302;1991.CrossRefPubMedGoogle Scholar
  3. 3.
    Chang CM, Shu HK, Kung HJ. Disease specificity of kinase domains: The src-encoded catalytic domain converts erbB into a sarcoma oncogene. Proc Natl Acad Sci USA 92:3928–3932;1995.PubMedGoogle Scholar
  4. 4.
    Edelman AM, Blumenthal DK, Krebs EG. Protein serine/threonine kinases. Annu Rev Biochem 56:567–613;1987.PubMedGoogle Scholar
  5. 5.
    Hanks SK. Homology probing: identification of cDNA clones encoding members of the protein-serine kinase family. Proc Natl Acad Sci USA 84:388–392;1987.PubMedGoogle Scholar
  6. 6.
    Hanks SK, Hunter T. The eukaryotic protein kinase superfamily. In: Hardie G, Hanks S, eds. The Protein Kinase Facts Book. San Diego, Academic Press, I:7–47;1995.Google Scholar
  7. 7.
    Hanks SK, Quinn AM, Hunter T. The protein kinase family conserved features and deduced phylogeny of the catalytic domains. Science 241:42–52;1988.Google Scholar
  8. 8.
    Hunter A, Cooper JA. Protein-tyrosine kinases. Annu Rev Biochem 54:897–930;1985.PubMedGoogle Scholar
  9. 9.
    Hunter T. A thousand and one protein kinases. Cell 50:823–829;1987.CrossRefPubMedGoogle Scholar
  10. 10.
    Hunter T. 1001 protein kinases redux-towards 2000. Semin Cell Biol 5:367–376;1994.CrossRefPubMedGoogle Scholar
  11. 11.
    Ishii H, Yoshida T, Oh H, Yoshida S, Terada M. A truncated K-k-sam product lacking the distal carboxyl-terminal portion provides a reduced level of autophosphorylation and greater resistance against induction of differentiation. Mol Cell Biol 15:3664–3671;1995.PubMedGoogle Scholar
  12. 12.
    Itoh H, Hattori Y, Sakamoto H, Ishii H, Kishi T, Sasaki H, Yoshida T, Koono M, Sugimura T, Terada M. Preferential alternative splicing in cancer generates a K-sam messenger RNA with higher transforming activity. Cancer Res 54:3237–3241;1994.PubMedGoogle Scholar
  13. 13.
    Iwase T, Tanaka M, Suzuki M, Naito Y, Sugimura H, Kino I. Identification of protein-tyrosine kinase genes preferentially expressed in embryo stomach and gastric cancer. Biochem Biophys Res Commun 194:698–705;1993.CrossRefPubMedGoogle Scholar
  14. 14.
    Jin LD, Meng CL, Han SH, Ding MJ, Chang TM, Chan TK, Shen KL. A study on production of monoclonal antibodies using SC-M1 cells as immunogen. Med Sci 8:17–25;1987.Google Scholar
  15. 15.
    Kameda T, Yasui W, Yoshida K, Tsujino T, Nakayama H, Ito M, Ito H, Tahara E. Expression of ERBB2 in human gastric carcinomas: Relationship between p185ERBB2 expression and the gene amplification. Cancer Res 50:8002–8009;1990.PubMedGoogle Scholar
  16. 16.
    Kuniyasu H, Xasui W, Kitadai Y, Yokozaki H, Ito H, Tahara E. Frequent amplification of c-met gene in scirrhous type stomach cancer. Biochem Biophys Res Commun 189:227–232;1992.CrossRefPubMedGoogle Scholar
  17. 17.
    Maniatis T, Fritsch E, Sambrook J. Molecular Cloning: A Laboratory Manual. ed 1. Cold Spring Harbor, Cold Spring Harbor Laboratory, 1982.Google Scholar
  18. 18.
    Mossie K, Jallal B, Alves F, Sures I, Plowman GD, Ullrich A. Colon carcinoma kinase-4 defines a new subclass of the receptor tyrosine kinase family. Oncogene 11:2179–2184;1995.PubMedGoogle Scholar
  19. 19.
    Robinson D, He F, Pretlow T, Kung H-J. A tyrosine kinase profile of prostate carcinoma. Proc Natl Acad Sci USA 93:5958–5962;1996.CrossRefPubMedGoogle Scholar
  20. 20.
    Schultz SJ, Nigg EA. Identification of 21 novel human protein kinases, including 3 members of a family related to the cell cycle regulator nima ofAspergillus nidulans. Cell Growth Different 4:821–830;1993.Google Scholar
  21. 21.
    Shimizu Y, Weidmann E, Iwatsuki S, Herberman RB, Whiteside TL. Characterization of human autotumor-reactive T-cell clones obtained from tumor-infiltrating lymphocytes in liver metastasis of gastric carcinoma. Cancer Res 51:6153–6162;1991.PubMedGoogle Scholar
  22. 22.
    Shu HK, Pelley RJ, Kung HJ. Tissue-specific transformation by epidermal growth factor receptor: A single point mutation within the ATP-binding pocket of the erbB product increases its intrinsic kinase activity and activates its sarcomagenic potential. Proc Natl Acad Sci USA 87:9103–9107;1990.PubMedGoogle Scholar
  23. 23.
    Takahashi T, Shirasawa T, Miyake K, Yahagi Y, Maruyama N, Kasahara N, Kawamura T, Matsumura O, Mitarai T, Sakai O. Protein tyrosine kinases expressed in glomeruli and cultured glomerular cells: FLT-1 and VEGF expression in renal mesangial cells. Biochem Biophys Res Commun 209:218–226;1995.CrossRefPubMedGoogle Scholar
  24. 24.
    Uchino S, Tsuda H, Maruyama K, Kinoshita T, Sasako M, Saito T, Kobayashi M, Hirohashi S. Overexpression of c-erbB-2 protein in gastric cancer. Its correlation with long-term survival of patients. Cancer 72:3179–3184;1993.PubMedGoogle Scholar
  25. 25.
    Wilks AF. Two putative protein-tyrosine kinases identified by application of the polymerase chain reaction. Proc Natl Acad Sci USA 86:1603–1607;1989.PubMedGoogle Scholar
  26. 26.
    Wilks AF, Kurban RR, Hovens CM, Ralph SJ. The application of the polymerase chain reaction to cloning members of the protein tyrosine kinase family. Gene 85:67–74;1989.CrossRefPubMedGoogle Scholar
  27. 27.
    Wu C-W, Hsieh M-C, Lo S-S, Tsay S-H, Lui W-Y, P'eng F-K. Relation of number of positive lymph nodes to the prognosis of patients with primary gastric adenocarcinoma. Gut 38:525–527;1996.PubMedGoogle Scholar
  28. 28.
    Wu C-W, Wang S-R, Chien S-L, Yeh T-H, Lian S-L, Shimizu N, Lui W-Y, P'eng F-K, Chi C-W. Regulation of arginase production by glucocorticoid in three human gastric cancer cell lines. Life Sci 51:1355–1361;1992.CrossRefPubMedGoogle Scholar
  29. 29.
    Yokota J, Yamamoto T, Miyajima N, Toyoshima K, Nomura N, Sakamoto H, Yoshida T, Terada M, Sugimura T. Genetic alterations of the c-erbB-2 oncogene occur frequently in tubular adenocarcinoma of the stomach and are often accompanied by amplification of the v-erb A homologue. Oncogene 2:283–287;1988.PubMedGoogle Scholar

Copyright information

© National Science Council 1998

Authors and Affiliations

  • Jyh-Shi Lin
    • 1
  • Chi-Wei Lu
    • 1
  • Chang-Jen Huang
    • 2
  • Peng-Fyn Wu
    • 1
  • Daniel Robinson
    • 3
  • Hsing-Jien Kung
    • 3
  • Chin-Wen Chi
    • 5
  • Chew-Wun Wu
    • 4
  • Wen-Kang Yang
    • 1
  • Jacqueline J. K. Whang-Peng
    • 1
  • Wen-chang Lin
    • 1
  1. 1.Institute of Biomedical SciencesAcademia Sinica and Clinical Cancer Center, National Health Research InstitutesTaipeiTaiwan, ROC
  2. 2.Institute of Biological ChemistryAcademia SinicaTaipeiTaiwan, ROC
  3. 3.Department of Molecular Biology and Microbiology, School of MedicineCase Western Reserve UniversityClevelandUSA
  4. 4.Department of SurgeryNational Yang-Ming UniversityTaipeiTaiwan, ROC
  5. 5.Department of Medical Research, Veterans General Hospital-Taipei and School of MedicineNational Yang-Ming UniversityTaipeiTaiwan, ROC

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