Skip to main content
SpringerLink
Log in

Gene expression in response to retinoic acid in novel human chromosome 21 monochromosomal cell hybrids

  • Brief Communication
  • Published:
Somatic Cell and Molecular Genetics

Abstract

To access a wide variety of expressed sequences from human chromosome 21 we have placed this chromosome into undifferentiated P19 mouse embryonic carcinoma cells. Cell lines resulting from these experiments have a range of morphologies and a wide variety of karyotypes. We have studied the retinoic acid response of five cell lines, compared to P19 cells, by observing three markers of retinoic acid induced P19 differentiation—cell morphology,RARα andWnt1 transcription. We see an ‘early’ retinoic acid response effect, however this response breaks down by the time the ‘late’ geneWnt1 would be transcribed in P19 cells. A highly responsive cell line will be useful for cloning expressed sequences from human chromosome 21 which are produced by early genes in retinoic acid inducible pathways, such as those involved in neurogenesis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Literature cited

  1. Nelson, D.L., Ledbetter, S.A., Corbo, L., Victoria, M.F., Ramirez-Solis, R., Webster, T.D., Ledbetter, D.H., and Caskey, C.T. (1989).Proc. Natl. Acad. Sci. U.S.A. 86:6686–6690.

    PubMed  Google Scholar 

  2. Cotter, F.E., Hampton, G.M., Nasipuri, S., Bodmer, W.F., and Young, B.D. (1990).Genomics.7:257–263.

    Article  PubMed  Google Scholar 

  3. Warburton, D., Gersen, S., Yu, M.-T., Jackson, C., Hadelin, B., and Housman, D. (1990).Genomics. 6:358–366.

    Article  PubMed  Google Scholar 

  4. Van Hul, W., Van Camp, G., Stuyver, L., Delabar, J.M., McInnis, M.G., Warren, A.C., Antonarakis, S.E., and Van Broeckhoven, C. (1993).Genomics. 15:626–630.

    Article  PubMed  Google Scholar 

  5. Jeggo, P.A., Hafezparast, M., Thompson, A.F., Broughton, B.C., Kaur, G.P., Zdzienicka, M.Z., and Athwal, R.S. (1992).Proc. Natl. Acad. Sci. U.S.A. 89:6423–6427.

    PubMed  Google Scholar 

  6. Sandhu, A.K., Hubbard, K., Kaur, G.P., Jha, K., Ozer, H.L., and Athwal, R.S. (1994).Proc. Natl. Acad. Sci. U.S.A. 91:5498–5502.

    PubMed  Google Scholar 

  7. Baens, M.B., Chaffanet, M., Cassiman, J.-J., van den Berghe, H., and Marynen, P. (1993).Genomics. 16:214–218.

    Article  PubMed  Google Scholar 

  8. Corbo, L., Maley, J.A., Nelson, D.L., and Caskey, C.T. (1990).Science. 249:652–655.

    PubMed  Google Scholar 

  9. Liu, P., Legerski, R., and Siciliano, M.J. (1989).Science. 246:813–815.

    PubMed  Google Scholar 

  10. McBurney, M.W., Jones-Villeneuve, E.M.V., Edwards, M.K.S., and Anderson, P.J. (1982).Nature. 299:165–167.

    Article  PubMed  Google Scholar 

  11. Jones-Villeneuve, E.M.V., McBurney, M.W., Rogers, K.A., and Kalnins, V.I. (1982).J. Cell Biol. 94:253–262.

    Article  PubMed  Google Scholar 

  12. McBurney, M.W., Reuhl, K.R., Ally, A.I., Nasipuri, S., Bell, J.C., and Craig, J. (1988).J. Neurosci. 8:1063–1073.

    PubMed  Google Scholar 

  13. Johnson, J.E., Zimmerman, K., Saito, T., and Anderson, D.J. (1992).Development. 114:75–87.

    PubMed  Google Scholar 

  14. Husmann, M., Görgen, I., Weisgerber, C., and Bitter-Suermann, D. (1989).Dev. Biol. 136:194–200.

    Article  PubMed  Google Scholar 

  15. Athwal, R.S., Smarsh, M., Searle, B.M., and Deo, S.S. (1985).Somat. Cell Mol. Genet. 11:177–187.

    Article  PubMed  Google Scholar 

  16. Mulligan, R.C., and Berg, P. (1981).Proc. Natl. Acad. Sci. U.S.A. 78:2072–2076.

    PubMed  Google Scholar 

  17. McBurney, M.W., and Rogers, B.J. (1982).Dev. Biol. 89:503–508.

    Article  PubMed  Google Scholar 

  18. Hartman, S.C., and Mulligan, R.C. (1988).Proc. Natl. Acad. Sci. U.S.A. 85:8047–8051.

    PubMed  Google Scholar 

  19. Killary, A.M., and Fournier, R.E.K. (1984).Cell. 38:523–534.

    Article  PubMed  Google Scholar 

  20. McNeill, C.A., and Brown, R.L. (1980).Proc. Natl. Acad. Sci. U.S.A. 77:5394–5398.

    PubMed  Google Scholar 

  21. Fournier, R.E.K., and Ruddle, F.H. (1977).Proc. Natl. Acad. Sci. U.S.A. 74:319–323.

    PubMed  Google Scholar 

  22. Siden, T.S., Kumlieu, J., Schwartz, C.E., and Rohme, D. (1992).Somat. Cell Mol. Genet. 18:33–44.

    Article  PubMed  Google Scholar 

  23. Ledbetter, S.A., Garcia-Heras, J., and Ledbetter, D.H. (1990).Genomics. 8:614–622.

    Article  PubMed  Google Scholar 

  24. Yulug, I.G., Egan, S.E., See, C.G., and Fisher, E.M.C. (1994).Genomics. 22:313–318.

    Article  PubMed  Google Scholar 

  25. Brand, N., Petkovich, M., Krust, A., Chambon, P., de The, H., Marchio, A., Tiollais, P., and Dejean, A. (1988).Nature. 332:850–853.

    Article  PubMed  Google Scholar 

  26. van Ooyen, A., and Nusse, R. (1984).Cell. 39:233–240.

    Article  PubMed  Google Scholar 

  27. Song, S., and Sui, C.-H. (1989).FEBS Letters. 256:51–54.

    Article  PubMed  Google Scholar 

  28. Schuuring, E., van Deemter, L., Roelink, H., and Nusse, R. (1989).Mol. Cell Biol. 9:1357–1361.

    PubMed  Google Scholar 

  29. Benham, F.J., Wiles, M.V., and Goodfellow, P.N., (1983).Mol. Cell Biol. 3:2259–2270.

    PubMed  Google Scholar 

  30. Walter, M.A., and Goodfellow, P.N. (1993).Trends Genet. 352:352–356.

    Article  Google Scholar 

  31. Cox, D.R., Burmeister, M., Price, E.R., Kim, S., and Myers, R.M. (1990).Science. 250:245–250.

    PubMed  Google Scholar 

  32. Antonarakis, S.E. (1993).Trends Genet. 9:142–148.

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry and Molecular Genetics, Imperial College School of Medicine at St. Mary's, W2 1PG, London, UK

    Isik G. Yulug & Elizabeth M. C. Fisher

  2. Division of Laboratory Medicine, Section of Hematology/Pathology, M.D. Anderson Cancer Center, 77098, Houston, Texas

    Ann M. Killary

  3. Fels Institute of Cancer Research and Molecular Biology, Temple University School of Medicine, 3420 N. Broad St., 19140, Philadelphia, Pennsylvania

    Arbansjit K. Sandhu & Raghbir S. Athwal

  4. The Galton Laboratory, MRC Human Biochemical Genetics Unit, Wolfson House, 4 Stephenson Way, NW1 2HE, London, UK

    Margaret Fox

Authors
  1. Isik G. Yulug
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ann M. Killary
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Arbansjit K. Sandhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Raghbir S. Athwal
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Margaret Fox
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Elizabeth M. C. Fisher
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yulug, I.G., Killary, A.M., Sandhu, A.K. et al. Gene expression in response to retinoic acid in novel human chromosome 21 monochromosomal cell hybrids. Somat Cell Mol Genet 21, 357–365 (1995). https://doi.org/10.1007/BF02257471

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02257471

Keywords

Search

Navigation