Summary
This paper deals with our experiences with glass-capillary chromatography in medical research. We reported earlier about chromatography of fatty acid methyl esters on a 50 m glass capillary, coated with FFAP. Now we have experimented with a 50 m Silar 10 C column, which gives even better results. The analysis of bile acids on 20 m columns, coated with OV-17, SE-54 or Silar-10 C yields good chromatograms and resolves the main bile acids by base line separation. Further, we are able to report the chromatography of steroid hormones extracted from human urine on a 50 m glass capillary coated with OV-101. Preliminary assessment can be made of the chromatography of the 20 major amino acids after butylation and acetylation on a 50 m Silar-10 C column. As an example of the analysis of vitamins we have chosen the chromatography of nicotinic acid and its isomer. In summary, we are able to show the wide variety of applications of the glass capillary column in medical research.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature
H. Jaeger, H. U. Klör, G. Blos andH. Ditschuneit, Chromatographia8, 507–510 (1975).
Applied Science Lab., State College, P.A., USA, Gas Chrom. Newsletter15 (6), 2–5 (1974).
E. R. Schiff, N. C. Small andJ. M. Dietschy, J. Clin. Invest.51, 1351–1362 (1972).
D. rachmilewitz andD. R. Saunders, Gastroenterology71, 82–86 (1976).
P. Back, Clin. Chim. Acta44, 199–207 (1973).
P. Eneroth, J. Lipid Res.4, 11–16 (1963).
T. A. Miettinen, E. H. Ahrens andJ. R. Grundy, J. Lipid Res.6, 411–421 (1965).
R. Blomstrand, Proc. Soc. Exptl. Biol. Med.107, 1226–1233 (1961).
T. Laatikainen andA. Hesso, Clin. Chim. Acta64, 63–68 (1975).
I. Makino, andJ. Sjövall, Anal. Lett.5, 341–346 (1972).
A. Stiehl, J. Clin. Invest.4, 59–63 (1974).
B. Brechbühler, L. Gay andH. Jaeger, II. International Symposium on Glass Capillary Chromatography, Hindelang, May 2nd–6th, 1977.
A. Ros andJ. F. Sommerville, J. Obst. Gyn. Brit. Comm.78, 1069–1107 (1971).
E. Bailey, M. Fenoughty andJ. R. Chapman, J. Chromatogr.96, 33–46 (1974).
H. L. Bradlow, Steroids11, 265–272 (1968).
I. E. Bush, The Chromatography of Steroids, 1961, Pergamon Press, Oxford, London, New York, Paris.
W. Blunck, Acta Endocrinol. (Copenhagen)59, Suppl. 134 (1968).
K. B. Erik-Nes andE. C. Horning, Gas-Phase Chromatography of Steroids, 1968, Springer Verlag, Berlin, Heidelberg, New York.
E. C. Horning, M. G. Horning, J. Szafranek, P. Van Hout, A. L. Gerinan, J. P. Thenot andC. D. Pfaffenberger, J. Chromatogr.91, 367–378 (1974).
K. Grob andG. Grob, Chromatographia5, 1–10 (1972).
E. Kovats, Helv. Chim. Acta41, 1915–1932 (1958).
W. J. A. Van Den Heuvel, W. L. Gardiner andE. C. Horning, J. Chromatogr.26, 287–395 (1967).
R. W. Zumwalt, D. Roach andC. W. Gehrke, J. Chromatogr.53, 171–193 (1970).
R. W. Zumwalt, K. Kuo andC. W. Gehrke, J. Chromatogr.57, 193–208 (1971).
W. B. Parsons, Arch. Intern. Med.107, 653–661 (1961).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Jaeger, H., Wagner, W., Homoki, J. et al. Glass-capillary chromatography in medical research. Chromatographia 10, 492–497 (1977). https://doi.org/10.1007/BF02257365
Issue Date:
DOI: https://doi.org/10.1007/BF02257365