Summary
A method is described for the routine preparation of high resolution stabilized phase open tubular (SPOT) columns. A finely dispersed siliceous material (Cab-O-Sil) is treated with benzyltriphenylphosphoniumchloride and dynamically coated onto the inside wall of glass capillary columns. Additional dynamics coating with a polar stationary phase yields thermostable columns of high efficiency. The columns show favourable adsorption properties and good long term stability. The mechanical stability of the stationary phase film is explained in terms of the rheological behaviour of concentrated disperisons. The designation PLOT columns appers inapplicable, and these columns will be referred to as SPOT columns. A theoretical model, describing band spreading in SPOT columns, is proposed and compared with the experimental results. Some applications in routine use and in ultra trace analysis are presented.
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Cramers, C.A., Vermeer, E.A. & Franken, J.J. Preparation and evaluation of polar stabilized phase open tubular (SPOT) columns. Chromatographia 10, 412–418 (1977). https://doi.org/10.1007/BF02257353
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DOI: https://doi.org/10.1007/BF02257353