Summary
For the title compounds 4,4′-DADPM, MOCA, 3,3′-DCB, 4-ADP and 4-ADPA listing on the EPA priority pollutant list, an analytical practicable, reliable, reproducible and sensitive procedure is required. Therefore a new method has been developed for the routine determination of these toxic aromatic amines in urine at the ppb level. The quantitative determination of amines is a suitable procedure of occupationally exposed persons. Urine sample preparation is done using simple liquid-liquid extraction followed by a precolumn enrichment (PRP1-material; Hamilton). Breakthrough measurements were done using an enrichment column packed with PRP1 material. The capacities of the studied amines ranged from 21.9mg/g to 96.6mg/g, while influent concentrations differed from 28.3mg/l to 332.0mg/l. The advantages of electrochemical detection regarding to selectivity and sensitivity are clearly indicated in this paper. Separation has been achieved applying reversed-phase-high-performance-liquid chromatography (LiChrosorb RP 18/5μm) followed by electrochemical or UV-detection. The detection limits employing an electrochemical detector at a potential of 1 V range from 2.2ng to 12.1ng. UV detection at 254 nm and 280 nm is about 10–100 times less sensitive. Recoveries from spiked water samples at the 5ppb levels were 75% to 96% respectively. The standard deviation of the developed procedure varies from 5.3% to 14%. Day-to-day repeatability is good.
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References
H. Blome, M. Hennig, Staub-Reinhalt. Luft44, 27 (1984).
D. Wolf, H. Blome, Staub-Reinhalt. Luft42, 101 (1982).
R. M. Riggin, C. C. Howard, D. R. Scott, J. Chromatogr. Sci.21, 321 (1983).
R. M. Riggin, C. C. Howard, Anal. Chem.51, 210 (1979).
J. R. Rice, P. T. Kissinger, Environ. Sci. Techniol.16, 263 (1982).
R. L. Jenkins, R. B. Baird, Bull. Environ. Contam. Toxicol.13, 436 (1975).
D. N. Armentroud, S. S. Cutie, J. Chromatogr. Sci.18, 370 (1980).
J. Lewalter, Jahresbericht 1985 der Bayer AG.
W. Will, K. Goßler, K. H. Schaller, Institut für Arbeits-und Sozialmedizin und der Poliklinik für Berufskrankheiten der Universität Erlangen-Nürnberg, ASP8, 201 (1981).
Chemical Hazards in the Workplace.G. Choundhary, ed., ACS Symposium Series 149, American Chemical Society, Washington, DC, 1981, pp. 413–27.
Analytische Methoden, Analysen in biol. Material,J. Angerer, K. H. Schaller, J. Lewalter, H.-G. Neumann, Aromatische Amine, Bd. 2, Seite D 6.
C. R. Nony, M. C. Bowman, Intern. J. Environ. Anal. Chem.5, 203 (1978).
M. C. Bowman, L. G. Rushing, Arch. Environ. Contam. Toxicol.6, 471 (1977).
M. C. Bowman, J. Assoc. Offic. Anal. Chem.61, 1253 (1978).
J. Lewalter, U. Korallus, Int. Arch. Occup. Environ. Health56, 179 (1985).
M. Tortoreto, P. Catalani, S. Paglilunga, J. Chromatogr.262, 367 (1983).
C. L. Holder, J. R. King, M. C. Bowman, J. Toxicol. and Environ. Hith.2, 111 (1976).
M. C. Bowman, J. R. King, C. L. Holder, Intern. J. Environ. Anal. Chem.4, 205 (1976).
C. R. Nony, M. C. Bowman, J. Chromatogr. Sci.18, 64 (1980).
J. R. Rice, P. T. Kissinger, J. Anal. Toxicol.3, 64 (1979).
J. W. Stanley, G. D. Newport, C. C. Weis, R. W. West, J. Liquid Chromatogr.1, 305 (1978).
L. Fishbein, Toxicol. Environ. Chem. Review3, 145 (1980).
DIN 38 402 Teil 5, Vom Wasser62, 331 (1984).
C. Vonderheid, V. Dammann, H. Krutz, Vom Wasser57, 59 (1981).
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Trippel-Schulte, P., Zeiske, J. & Kettrup, A. Trace analysis of selected benzidine and diaminodiphenylmethane derivates in urine by means of liquid chromatography using precolumn sample preconcentration, UV and electrochemical detection. Chromatographia 22, 138–146 (1986). https://doi.org/10.1007/BF02257315
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DOI: https://doi.org/10.1007/BF02257315