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A high-performance liquid chromatographic method for the measurement of amiodarone and its main metabolite (desethylamiodarone) in plasma samples from patients treated withCordarex ®

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Summary

A procedure for the rapid, quantitative isolation of amiodarone and its main metabolite (desethylamiodarone) from plasma with SEP-PAK™ silica cartridges is described together with a sensitive high performance liquid chromatographic assay for the quantitative determination of the drugs.

The recovery of amiodarone and its metabolite was greater than 80% over an investigated range of 0.1–5μg/ml of plasma and the limit of quantitation by the assay was 50 ng/ml of plasma.

The column extraction of amiodarone and its metabolite coupled with the chromatographic versatility of the method make it suited for either detailed pharmacokinetic studies and routine plasma analysis of amiodarone.

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Authors and Affiliations

  1. Department of Clinical Biochemistry, Diabetic Research Institute, University of Düsseldorf, Auf'm Hennekamp 65, D-4000, Düsseldorf 1, Federal Republic of Germany

    F. Susanto, S. Humfeld & H. Reinauer

Authors
  1. F. Susanto
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  2. S. Humfeld
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  3. H. Reinauer
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Susanto, F., Humfeld, S. & Reinauer, H. A high-performance liquid chromatographic method for the measurement of amiodarone and its main metabolite (desethylamiodarone) in plasma samples from patients treated withCordarex ® . Chromatographia 22, 135–137 (1986). https://doi.org/10.1007/BF02257314

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  • DOI: https://doi.org/10.1007/BF02257314

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