Summary
The interconversion between the α- and β-isomers and the multiple-peaks of dihydroartemisinine (dihydroqinghaosu) were investigated in reversed-phase, highperformance liquid chromatography. A convenient method using the Gaussian distribution function instead of the Schmidt graphical method for the theoretical calculation of the “diffuse” probability curves versus the fraction of the dead time on column as β form was introduced. Based on the theoretical probability curves and the experimental multiple-peak chomatograms, the effects of temperature and flow velocity on the accuracy and precision of analysis of dihydroartemisinine are indicated and the activation energy of the interconversion between α- and β-isomers of dihydroartemisinine is estimated.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Li Ying, Yu Peiling, Chen Yixin, Li Liangquan, Acta Pharmaceutica Sinica16, 429 (1981).
R. A. Keller, G. C. Giddings, J. Chromatogr.3, 205 (1960).
J. Crank, in “The Mathematics of Diffusion”, Clarendon Press, Oxford (1956).
J. F. K. Huber, in “Instrumentation for High-performance Liquid Chromatography”, Elsevier, Amsterdam (1978).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shishan, Z. Equilibrium between α- and β-isomers of dihydroartemisinine and its multiple-peaks in high-performance liquid chromatography. Chromatographia 22, 77–80 (1986). https://doi.org/10.1007/BF02257303
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02257303