Summary
The application of a simple reversed-phase, HPLC-UV method for the determination of the contact herbicide pyridate and its main metabolite 3-phenyl-4-hydroxy-6-chloropyridazine-known as CL-9673-in green cereals, grains and straw is described. The method is based on a simple extraction, separation of pyridate from its metabolite, hydrolysis of pyridate to CL-9673, high-performance liquid chromatographic separation of CL-9673 in both fractions using only one reversed phase column and UV-detection.
The method is sensitive down to 3 ngg−1 CL-9673 in green plants and grain and 5ngg−1 in straw.
In the range 0.05–1.0mgkg−1 recoveries are between 73.7% and 109% from green plants, between 77.7% and 102% from grains and between 77.3% and 87.5% from straw.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
J. F. K. Huber, I. Fogy, C. Fioresi, chromatographia13, 408 (1980).
W. Perkow, Wirksubstanzen der Pflanzenschutz- und Schädlingsbekämpfungsmittel,4. Erg. Lfg., 1980 Verlag Paul Parey.
W. Laindner, H. Ruckendorfer, Graz University, Austria (1981) unpublished.
W. Landvoigt, H. Malissa jr., K. Winsauer, Mikrochim. Acta Wien 1982 II, 199–214.
H. Malissa jr., W. Buchberger, E. Landgraph, K. Winsauer, Mikrochim. Acta Wien 1984 1, 127–142.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Alawi, M.A. Determination of pyridate and its main metabolite CL-9673 in plant materials. Chromatographia 22, 40–42 (1986). https://doi.org/10.1007/BF02257294
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02257294