Summary
Rf values of twenty therapeutic sulfonamides have been estimated in seven solvent systems. Based on these data a simple identification scheme was developed demanding only three solvent systems for complete separation.
Quantification of corresponding fluorescamine derivatives at nanogram level was performed by the use of an TLC-scanner configured online with a microcomputer. Mathematical treatment of standard curves using the computer program Curve Fitter are discussed. Validity and reproducibility of results are compared with data obtained by HPLC and show good correlation.
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Knupp, G., Pollmann, H. & Jonas, D. An improved HPTLC method for the rapid identification and quantification of sulfonamides. Chromatographia 22, 21–24 (1986). https://doi.org/10.1007/BF02257291
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DOI: https://doi.org/10.1007/BF02257291