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Hepatoprotective effects ofArctium lappa linne on liver injuries induced by chronic ethanol consumption and potentiated by carbon tetrachloride


Arctium lappa Linne (burdock) is a perennial herb which is popularly cultivated as a vegetable. In order to evaluate its hepatoprotective effects, a group of rats (n=10) was fed a liquid ethanol diet (4 g of absolute ethanol/80 ml of liquid basal diet) for 28 days and another group (n=10) received a single intraperitoneal injection of 0.5 ml/kg carbon tetrachloride (CCI4) in order to potentiate the liver damage on the 21st day (1 day before the beginning ofA. lappa treatment). Control group rats were given a liquid basal diet which did not contain absolute ethanol. When 300 mg/kgA. lappa was administered orally 3 times per day in both the 1-day and 7-day treatment groups, some biochemical and histopathological parameters were significantly altered, both in the ethanol group and the groups receiving ethanol supplemented with CCI4.A. lappa significantly improved various pathological and biochemical parameters which were worsened by ethanol plus CCI4-induced liver damage, such as the ethanol plus CCI4-induced decreases in total cytochrome P-450 content and NADPH-cytochrome c reductase activity, increases in serum triglyceride levels and lipid peroxidation (the deleterious peroxidative and toxic malondialdehyde metabolite may be produced in quantity) and elevation of serum transaminase levels. It could even restore the glutathione content and affect the histopathological lesions. These results tended to imply that the hepatotoxicity induced by ethanol and potentiated by CCI4 could be alleviated with 1 and 7 days ofA. lappa treatment. The hepatoprotective mechanism ofA. lappa could be attributed, at least in part, to its antioxidative activity, which decreases the oxidative stress of hepatocytes, or to other unknown protective mechanism(s).

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  1. 1

    Bardina LR, Satanovskaia VI, Pron'ko PS, Kuz'mich AB. Activity of the enzymes of ethanol and acetaldehyde metabolism in rats with varying initial sensitivity to alcohol (in Russian). Ukr Biokhim Zh 69:94–99;1997.

    Google Scholar 

  2. 2

    Bergmeyer HU, Scheibe P, Wahlefeld AW. Optimization of methods for aspartate aminotransferase and alanine aminotransferase. Clin Chem 24:58–73;1978.

    Google Scholar 

  3. 3

    Bondy SC, Naderi S. Contribution of hepatic cytochrome P450 systems to the generation of reactive oxygen species. Biochem Pharmacol 48:155–159;1994.

    Google Scholar 

  4. 4

    Bucolo G, David H. Quantitative determination of serum triglyceride by the use of enzymes. Clin Chem 19:476–482;1973.

    Google Scholar 

  5. 5

    Buege JA, Aust SD. Microsomal lipid peroxidation. Methods Enzymol 52:302–322;1978.

    Google Scholar 

  6. 6

    Comporti M. Lipid peroxidation and cellular damage in toxic liver injury. Lab Invest 53:599–623;1985.

    Google Scholar 

  7. 7

    Comporti M. Three models of free radical-induced cell injury. Chem Biol Interact 72:1–56;1989.

    Google Scholar 

  8. 8

    Cornish HH, Adefuin J. Potentiation of carbon tetrachloride toxicity by aliphatic alcohols. Arch Environ Health 14:447–449;1967.

    Google Scholar 

  9. 9

    Cronholm T. Hydrogen transfer between ethanol molecules during oxidoreduction in vivo. Biochem J 229:315–322;1985.

    Google Scholar 

  10. 10

    Cronholm T, Jones AW, Skagerberg S. Mechanism and regulation of ethanol elimination in humans: Intermolecular hydrogen transfer and oxidoreduction in vivo. Alcohol Clin Exp Res 12:683–686;1988.

    Google Scholar 

  11. 11

    DeCarli LM, Lieber CS. Fatty liver in the rat after prolonged intake of ethanol with a nutritionally adequate new liquid diet. J Nutr 91:331–336;1967.

    Google Scholar 

  12. 12

    Domschke S, Domschke W, Lieber CS. Hepatic redox state: Attenuation of the acute effects of ethanol induced by chronic ethanol consumption. Life Sci 15:1327–1334;1974.

    Google Scholar 

  13. 13

    Forsander OA, Maenpaa PH, Salaspuro MP. Influence of ethanol on the lactate/pyruvate and beta-hydroxybutiate/acetoacetate ratios in rat liver experiments. Acta Chem Scand 19:1770–1771;1965.

    Google Scholar 

  14. 14

    Ikatsu H, Okino T, Nakajima T. Ethanol and food deprivation induced enhancement of hepatotoxicity in rats given carbon tetrachloride at low concentration. Br J Ind Med 48:636–642;1991.

    Google Scholar 

  15. 15

    Ingelman-Sundberg M, Johansson I, Yin H, Terelius Y, Eliasson E, Clot P, Albano E. Ethanol-inducible cytochrome P4502E1: Genetic polymorphism, regulation, and possible role in the etiology of alcohol-induced liver disease. Alcohol 10:447–452;1993.

    Google Scholar 

  16. 16

    Iwakami S, Wu JB, Ebizuka Y, Sankawa U. Platelet activating factor (PAF) antagonists contained in medicinal plants: Lignans and sesquiterpenes. Chem Pharm Bull (Tokyo) 40:1196–1198;1992.

    Google Scholar 

  17. 17

    Kan WS. Pharmaceutical Botany. Taipei, National Research Institute of Chinese Medicine, 1981.

  18. 18

    Koop DR, Morgan ET, Tarr GE, Coon MJ. Purification and characterization of a unique isozyme of cytochrome P-450 from liver microsomes of ethanol-treated rabbits. J Biol Chem 257:8472–8480;1982.

    Google Scholar 

  19. 19

    Lieber CS. Biochemical factors in alcoholic liver disease. Semin Liver Dis 13:136–153;1993.

    Google Scholar 

  20. 20

    Lieber CS. Alcohol and the liver: 1994 update. Gastroenterology 106:1085–1105;1994.

    Google Scholar 

  21. 21

    Lieber CS, DeCarli LM. Quantitative relationship between amount of dietary fat and severity of alcoholic fatty liver. Am J Clin Nutr 23:474–478;1970.

    Google Scholar 

  22. 22

    Lin CC, Lu JM, Yang JJ, Chuang SC, Ujiie T. Anti-inflammatory and radical scavenge effects ofArctium lappa. Am J Chin Med 24:127–137;1996.

    Google Scholar 

  23. 23

    Lin SC, Chung TC, Lin CC, Ueng TH, Lin YH, Lin SY, Wang LY. Hepatoprotective effects ofArctium lappa on carbon tetrachloride-and acetaminophen-induced liver damage. Am J Chin Med 28:163–173;2000.

    Google Scholar 

  24. 24

    Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275;1951.

    Google Scholar 

  25. 25

    Morita K, Kada T, Namiki M. A desmutagenic factor isolated from burdock (Arctium lappa Linne). Mutat Res 129:25–31;1984.

    Google Scholar 

  26. 26

    Morita T, Ebihara K, Kiriyama S. Dietary fiber and fat-derivatives prevent mineral oil toxicity in rats by the same mechanism. J Nutr 123:1575–1585;1993.

    Google Scholar 

  27. 27

    Nose M, Fujimoto T, Takeda T, Nishibe S, Ogihara Y. Structural transformation of lignan compounds in rat gastrointestinal tract. Planta Med 58:520–523;1992.

    Google Scholar 

  28. 28

    Omura T, Sato R. The carbon monoxide-binding pigment of liver microsomes. J Biol Chem 239:2370–2385;1964.

    Google Scholar 

  29. 29

    Phillips AH, Langdon RG. Hepatic triphosphopyridine nucleotide- cytochrome c reductase: Isolation, characterization and kinetic studies. J Biol Chem 37:2652–2660;1962.

    Google Scholar 

  30. 30

    Recknagel RO, Glende EA Jr, Dolak JA, Waller RL. Mechanisms of carbon tetrachloride toxicity. Pharmacol Ther 43:139–154;1989.

    Google Scholar 

  31. 31

    Shaw S, Rubin KP, Lieber CS. Depressed hepatic glutathione and increased diene conjugates in alcoholic liver disease. Evidence of lipid peroxidation. Dig Dis Sci 28:585–589;1983.

    Google Scholar 

  32. 32

    Slater TF, Cheeseman KH, Ingold KU. Carbon tetrachloride toxicity as a model for studying free-radical mediated liver injury. Philos Trans R Soc Lond B Biol Sci 311:633–645;1985.

    Google Scholar 

  33. 33

    Tietze F. Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: Applications to mammalian blood and other tissues. Anal Biochem 27:502–522;1969.

    Google Scholar 

  34. 34

    Ueng TH, Moore L, Elves RG, Alvares AP. Isopropanol enhancement of cytochrome P-450-dependent monooxygenase activities and its effects on carbon tetrachloride intoxication. Toxicol Appl Pharmacol 71:204–214;1983.

    Google Scholar 

  35. 35

    Wong SH, Knight JA, Hopfer SM, Zaharia O, Leach CN Jr, Sunderman FW Jr. Lipoperoxides in plasma as measured by liquid-chromatographic separation of malondialdehydethiobarbituric acid adduct. Clin Chem 33:214–220;1987.

    Google Scholar 

  36. 36

    Yuda Y, Tanaka J, Hirano F, Igarashi K, Satoh T. Participation of lipid peroxidation in rat pertussis vaccine pleurisy. 3. Thiobarbituric acid (TBA) reactant and lysosomal enzyme. Chem Pharm Bull (Tokyo) 39:505–506;1991.

    Google Scholar 

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Lin, SC., Lin, CH., Lin, CC. et al. Hepatoprotective effects ofArctium lappa linne on liver injuries induced by chronic ethanol consumption and potentiated by carbon tetrachloride. J Biomed Sci 9, 401–409 (2002).

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Key Words

  • Arctium lappa
  • Ethanol
  • Carbon tetrachloride
  • Liver injury