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Journal of Biomedical Science

, Volume 6, Issue 1, pp 53–63 | Cite as

Peptide loading in the endoplasmic reticulum accelerates trafficking of peptide: MHC class II complexes in B cells

  • Stanislaw Morkowski
  • Graça Raposo
  • Hans J. Geuze
  • Alexander Y. Rudensky
Original Paper

Abstract

In a combination of biochemical and immunoelectron-microscopical approaches we studied intracellular trafficking and localization of the endoplasmic-reticulum (ER)-formed complexes of murine MHC class II molecule I-Ab and an antigenic peptide Eα52–68 covalently linked to its β-chain. The association with the peptide in the ER leads to sharp acceleration of the intracellular trafficking of the complexes to the plasma membrane. Within the cells, Eα52–68:I-Ab complexes accumulate in the multivesicular MHC class II compartment (MIIC), but not in denser multilaminar or intermediate type MIICs. The changes in the trafficking of ER-formed complexes result solely from the presence of the tethered peptide, since wild-type class II molecules traffic similarly in bare lymphocyte syndrome cells and in wild-type antigen-presenting cells.

Key Words

MHC class II Invariant chain Class II:peptide complexes Intracellular trafficking 

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Copyright information

© National Science Council 1999

Authors and Affiliations

  • Stanislaw Morkowski
    • 1
  • Graça Raposo
    • 2
  • Hans J. Geuze
    • 2
  • Alexander Y. Rudensky
    • 3
    • 1
  1. 1.The Department of ImmunologyUniversity of Washington School of MedicineSeattleUSA
  2. 2.Department of Cell Biology and Institute of BiomembranesUniversity of Utrecht School of MedicineUtrechtThe Netherlands
  3. 3.Howard Hughes Medical InstituteUniversity of WashingtonSeattleUSA

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