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Journal of Biomedical Science

, Volume 7, Issue 1, pp 51–57 | Cite as

DNA vaccination using the fragment C of botulinum neurotoxin type A provided protective immunity in mice

  • Rong-Hwa Shyu
  • Men-Fang Shaio
  • Shiao-Shek Tang
  • Huey-Fen Shyu
  • Chi-Feng Lee
  • Meng-Hung Tsai
  • Jason E. Smith
  • Hsin-Hsien Huang
  • Jiunn-Jye Wey
  • Jan-Ling Huang
  • Hsin-Hou Chang
Original Paper

Abstract

Botulinum neurotoxin (BoNT) is one of the most toxic substances known to produce severe neuromuscular paralysis. The currently used vaccine is prepared mainly from biohazardous toxins. Thus, we studied an alternative method and demonstrated that DNA immunization provided sufficient protection against botulism in a murine model. A plasmid of pBoNT/A-Hc, which encodes the fragment C gene of type A botulinum neurotoxin, was constructed and fused with an Igκ leader sequence under the control of a human cytomegalovirus promoter. After 10 cycles of DNA inoculation with this plasmid, mice survived lethal doses of type A botulinum neurotoxin challenges. Immunized mice also elicited cross-protection to the challenges of type E botulinum neurotoxin. This is the first study demonstrating the potential use of DNA vaccination for botulinum neurotoxins.

Key Words

DNA vaccine Botulinum neurotoxin Fragment C 

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Copyright information

© National Science Council 2000

Authors and Affiliations

  • Rong-Hwa Shyu
    • 2
  • Men-Fang Shaio
    • 2
  • Shiao-Shek Tang
    • 2
  • Huey-Fen Shyu
    • 2
  • Chi-Feng Lee
    • 2
  • Meng-Hung Tsai
    • 2
  • Jason E. Smith
    • 1
  • Hsin-Hsien Huang
    • 2
  • Jiunn-Jye Wey
    • 2
  • Jan-Ling Huang
    • 2
  • Hsin-Hou Chang
    • 2
  1. 1.Department of BiologyYale UniversityNew HavenUSA
  2. 2.Institute of Preventive MedicineNational Defense Medical CenterTaipeiTaiwan (Republic of China)

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