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Somatic Cell and Molecular Genetics

, Volume 20, Issue 6, pp 529–540 | Cite as

Unstable integration of transfected DNAs into embryonal carcinoma cells

  • Michael W. McBurney
  • Susan Fournier
  • Prabha K. Schmidt-Kastner
  • Karen Jardine
  • Jane Craig
Article

Abstract

Plasmid DNA can be efficiently transfected into embryonal carcinoma cells but it is difficult to isolate clones of cells stably expressing genes present on the transfected plasmids. Even in clonal populations derived from transfected cells, the introduced genes are expressed in some but not all cells. Cotransfection with a region of thePgk-1 gene results in more efficient, stable cotransformation due to increased numbers of copies of the transfected plasmids integrated into the genomic DNA. ThePgK-1 genomic sequences did not allow the plasmid DNA to replicate autonomously but seemed to enhance the ligation of transfected plasmids before their integration into the host genome. Our results suggest a model in which the plasmid DNAs are able to integrate and subsequently excise from the host genome by recombination events enhanced by transcription through the tandemly repeated sequences of the transfected plasmids.

Keywords

Carcinoma Carcinoma Cell Genomic Sequence Recombination Event Host Genome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • Michael W. McBurney
    • 1
  • Susan Fournier
    • 1
  • Prabha K. Schmidt-Kastner
    • 1
  • Karen Jardine
    • 1
  • Jane Craig
    • 1
  1. 1.Department of MedicineUniversity of OttawaOttawaCanada

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