Somatic Cell and Molecular Genetics

, Volume 20, Issue 6, pp 515–528 | Cite as

Intragenic regions of the murinePgk-1 locus enhance integration of transfected DNAs into genomes of embryonal carcinoma cells

  • Michael W. McBurney
  • Susan Fournier
  • Karen Jardine
  • Leslie Sutherland


Introduction of recombinant genes into mammalian cells in culture has been an important procedure in establishing the molecular mechanisms of various cellular processes. The efficiency with which plasmid borne recombinant genes are expressed following stable integration into genomes of embryonal carcinoma cells is low. Using the P19 embryonal carcinoma cells as recipients, we found that constructs carrying the promoter and intragenic regions of the murinePgk-1 gene were expressed with high efficiency. This elevated expression was associated with increased numbers of copies of the transfected plasmid DNA stably associated with the genomes of recipient cells. The elevated plasmid copy numbers may result from enhanced ligation of transfected plasmids because cotransfected plasmids were also integrated in increased numbers. The enhanced integration and expression of transfected plasmids required active transcription through an intragenic region ofPgk-1, perhaps resulting in more recombinogenic plasmid DNAs.


Carcinoma Molecular Mechanism Mammalian Cell Cellular Process Active Transcription 
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Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • Michael W. McBurney
    • 1
  • Susan Fournier
    • 1
  • Karen Jardine
    • 1
  • Leslie Sutherland
    • 1
  1. 1.Department of MedicineUniversity of OttawaOttawaCanada

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