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Somatic Cell and Molecular Genetics

, Volume 20, Issue 6, pp 493–504 | Cite as

Characterization of spontaneous and induced mutations in SV40-transformed normal and ataxia telangiectasia cell lines

  • Christiane Röscheisen
  • Sabine Haupter
  • Ulrich Zechner
  • Günter Speit
Article

Abstract

Spontaneous and induced mutations at the HPRT locus were analyzed in one normal (MRC5CV1) and one ataxia telangiectasia (AT5BIVA) SV40-transformed cell line derived from male donors. Multiplex PCR and Southern analyses revealed a high frequency of spontaneous deletion mutations that may be a consequence of the SV40 transformation. Four mutagens (ethyl methanesulfonate, bromodeoxyuridine, bleomycin, adriamycin), which differ in their types of primary DNA lesions, caused specific patterns of mutations. By using fluorescence in situ hybridization (FISH) techniques, we were able to show that more than 90% of the AT5BIVA cells contained two X chromosomes with HPRT alleles, while in more than 90% of the MRC5CV1 cells genomic hemizygosity for the HPRT gene was found. Taking into account these findings we found that the AT5BIVA cell line possesses spontaneous hypermutability as well as hypersensitivity and hypermutability to bleomycin (BLM) and adriamycin (AM). Both mutagens induced deletion mutations in both cell lines, but more complex mutations and larger deletions were found in AT5BIVA cells.

Keywords

Deletion Mutation Bleomycin Adriamycin Methanesulfonate Induce Mutation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Pulbishing Corporation 1994

Authors and Affiliations

  • Christiane Röscheisen
    • 1
  • Sabine Haupter
    • 1
  • Ulrich Zechner
    • 1
  • Günter Speit
    • 1
  1. 1.Abteilung Klinische GenetikUniversität UlmUlmGermany

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