Skip to main content
SpringerLink
Log in

Characterization of cDNAs coding for glucose phosphate isomerase and phosphoglycerate kinase in chinese hamster ovary cell line CHO-K1 and identification of defects in R1.1.7, a glycolysis-deficient variant of CHO-K1

  • Brief Communication
  • Published:
Somatic Cell and Molecular Genetics

Abstract

Full-length cDNAs for glucose phosphate isomerase (GPI) and phosphoglycerate kinase (PGK) of the Chinese hamster ovary cell line CHO-K1 have been characterized using RT-PCR and cycle sequencing of the PCR-amplified templates. Mutations in both genes have been identified in a glycolysis-deficient chinese hamster ovary cell line, R1.1. 7, derived from CHO-K1 cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Literature Cited

  1. Faik, P., and Morgan, M.J. (1977).Cell. Biol. Int. Rep. 1:555–562.

    PubMed  Google Scholar 

  2. Faik, P., and Morgan, M.J. (1977).Cell. Biol. Int. Rep. 1:563–570.

    PubMed  Google Scholar 

  3. Morgan, M.J., and Faik, P. (1980).Cell. Biol. Int. Rep. 14:121–127.

    Article  Google Scholar 

  4. Morgan, M.J., and Faik, P. (1981).Biosci. Rep. 1:669–686.

    Article  PubMed  Google Scholar 

  5. Morgan, M.J., Bownness, K.M., and Faik, P. (1981b).Biosci. Rep. 1:811–817.

    Article  PubMed  Google Scholar 

  6. Faik, P., Walker, J.I.H., and Morgan, M.J. (1988).Somat. Cell Mol. Genet. 14:287–292.

    Article  PubMed  Google Scholar 

  7. Southern, E.M. (1975).J. Mol. Biol. 98:503–517.

    PubMed  Google Scholar 

  8. Ogden, R.C., and Adams, D.A. (1987).Methods Enzymol. 152:61–90.

    PubMed  Google Scholar 

  9. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989). InMolecular Cloning: A Laboratory Manual, (Cold Spring Harbor, Laboratory, Cold Spring Harbor, New York).

    Google Scholar 

  10. Feinberg, A.P., and Vogelstein, B. (1983).Anal. Biochem. 132:6–13.

    Article  PubMed  Google Scholar 

  11. Benton, W.D., and Davis, R.W. (1977).Science 196:180–182.

    PubMed  Google Scholar 

  12. Patterson, A.T., and Dean, A. (1987).Nucleic Acids. Res. 15:6298.

    PubMed  Google Scholar 

  13. Fothergill-Gilmore, L.A. (1986).TIBS 11:47–55.

    Google Scholar 

  14. Frohman, M.A., Dush, M.K., and Martin, G.R. (1988).Proc. Natl. Acad. Sci. U.S.A. 85:8998–9002.

    PubMed  Google Scholar 

  15. Rawson, S.J. (1986). The Chinese hamster phosphoglycerate kinase gene family.PhD thesis. University of Leicester.

  16. Faik, P., Morgan, M.J., Naftalin, R.J., and Rist, R. (1989).Biochem. J. 260:153–155.

    PubMed  Google Scholar 

  17. Kao, F.T. and Puck, T.T. (1969).J. Cell Physiol. 74:245–257.

    Article  PubMed  Google Scholar 

  18. Deaven, L.L., and Peterson, D.F. (1973).Chromosoma 41:129–144.

    Article  PubMed  Google Scholar 

  19. Siciliano, M.J., Stallings, R.L., Adair, G.M., Humphrey, R.M., and Siciliano, J. (1983).Cytogenet. Cell. Genet. 35:15–20.

    PubMed  Google Scholar 

  20. Worton, R.J., Ho, C.C., and Duff, C. (1977).Somat. Cell Genet. 3:27–45.

    Article  PubMed  Google Scholar 

  21. Faik, P., Walker, J.I.H., and Morgan, M.J. (1994).Genomics 21:122–127

    Article  PubMed  Google Scholar 

  22. Bryant, T.N., Watson, H.C., and Wendell, P.L. (1974).Nature 247:14–17.

    Article  PubMed  Google Scholar 

  23. Blake, C.C.F., Evans, P.R., and Scopes, R.K. (1972).Nature (London), New Biol. 235:195–198.

    Google Scholar 

  24. Watson, H.C., Walker, N.P.C., Shaw, P.J., Bryant, T.N., Wendell, P.L., Fothergill, L.A., Perkins, R.E., Conroy, S.C., Dobson, M.J., Tuite, M.F., Kingsman, A.J., and Kingsman, S.M. (1982).EMBO J. 1:1635–1640.

    PubMed  Google Scholar 

  25. Banks, D.R., Blake, C.C.F., Evans, P.R., Haser, R., Rice, D.W., Hardy, G.W., Merret, M., and Phillips, A.W. (1979)Nature 279:773–777.

    Article  PubMed  Google Scholar 

  26. Harlos, K., Vas, M., and Blake, C.F. (1992).Proteins 12:133–144.

    Article  PubMed  Google Scholar 

  27. Joao, C.H., and Williams, R.J.P. (1993).Eur. J. Biochem. 216:1–18.

    Article  PubMed  Google Scholar 

  28. Anderson, C.M., Zucker, F.H., and Steitz, T.A. (1979).Science 204:375–380.

    PubMed  Google Scholar 

  29. Barber, M.D., Gamblin, S.J., Watson, H.C., and Littlechild, J.A. (1993).FEBS Lett. 320:193–197.

    Article  PubMed  Google Scholar 

  30. Fothergill, L.A., and Michels, P.A.M. (1993).Prog. Biophys. Mol. Biol. 59:105–235.

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Wellcome Laboratory For Molecular Genetics, Division of Biochemistry and Molecular Biology, UMDS, Guy's Campus, London Bridge, SE 1 9RT, London, UK

    Aycan F. Hassan-Walker, Michael J. Morgan & Pelin Faik

Authors
  1. Aycan F. Hassan-Walker
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Michael J. Morgan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Pelin Faik
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hassan-Walker, A.F., Morgan, M.J. & Faik, P. Characterization of cDNAs coding for glucose phosphate isomerase and phosphoglycerate kinase in chinese hamster ovary cell line CHO-K1 and identification of defects in R1.1.7, a glycolysis-deficient variant of CHO-K1. Somat Cell Mol Genet 21, 75–81 (1995). https://doi.org/10.1007/BF02255824

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02255824

Keywords

Search

Navigation