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Modulation ofAPRT transcription by altering spacing betweenCis-regulatory elements

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Somatic Cell and Molecular Genetics

Abstract

Three regulatory regions on the promoter of CHO adenine phosphoribosyltransferase gene (APRT) were identified. Spacing constraints between these regions were analyzed. With normal spacing, region II (−33 to +19), which separates regions I (−101 to −53) and III (+56 to +85), is critical forAPRT transcription. However, when regions I and III are artificially placed in proximity to each other (region II deleted), they are able to drive transcription as efficiently as the wild-typeAPRT promoter. Neither region I nor III alone is sufficient for efficient transcription. As the spacing between the two regions increases, the transcription decreases. Region I may activate transcription in two ways: through a stringent sequence-specific manner (as in the transcription mediated by regions I and III) and through a manner with relaxed requirement for sequence specificity (as in the transcription from the wild-typeAPRT promoter).

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Author notes

  1. Bin-Ru She

    Present address: RBIO, Salk Institute, 10010 N. Torrey Pines Rd., 92037, La Jolla, California

  2. Joel E. Schaley

    Present address: Department of Microbiology, SUNY, 11794-5222, Stony Brook, New York

Authors and Affiliations

  1. Department of Biology, Indiana University, 47405, Bloomington, Indiana

    Bin-Ru She, Joel E. Schaley & Milton W. Taylor

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  1. Bin-Ru She
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  2. Joel E. Schaley
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  3. Milton W. Taylor
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She, BR., Schaley, J.E. & Taylor, M.W. Modulation ofAPRT transcription by altering spacing betweenCis-regulatory elements. Somat Cell Mol Genet 21, 43–50 (1995). https://doi.org/10.1007/BF02255821

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  • DOI: https://doi.org/10.1007/BF02255821

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