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Persistence during G1 of gamma ray- or mitomycin C-induced lesions eliciting SCE in murine salivary gland cells in vivo

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Somatic Cell and Molecular Genetics

Abstract

The efficiency of mitomycin C or gamma rays to induce SCE in early or late G1 was determined in synchronized murine salivary gland cells in vivo, as a measure of the capacity of this tissue to repair the lesions involved in SCE formation before S. The SCE frequencies induced by MMC in the first division (before BrdU incorporation) were significantly lower in the early G1 compared to the late G1, indicating some repair of SCE-inducing lesions. In the second division (after BrdU incorporation), there was no difference between SCE induced in early and late G1, indicating that MMC-induced lesions in such conditions are very persistent and not repairable during G1. The radio induced SCE frequency at early G1 was significantly lower than that observed in late G1, in cells irradiated after BrdU incorporation, suggesting that half of gamma ray-induced DNA lesions that elicit SCEs were repaired.

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Literature Cited

  1. Wolff, S., Bodycote, J., and Painter, R.B. (1974).Mutat. Res. 25:73–81.

    Article  PubMed  Google Scholar 

  2. Kato, H. (1980).Cancer Genet. Cytogenet. 2:69–77.

    Article  Google Scholar 

  3. Conner, M.K., Cheng, M., and Biegel, J.A. (1984).Mutat. Res. 126:35–46.

    PubMed  Google Scholar 

  4. Ishii, Y., and Bender, M.A. (1978).Mutat. Res. 51:411–418.

    PubMed  Google Scholar 

  5. Popescu, N.C., Amsbaugh, S.C., and DiPaolo, J.A. (1985).Carcinogenesis 6:1627–1630.

    PubMed  Google Scholar 

  6. Stetka, D.G., Minkler, J., and Carrano, A.V. (1978).Mutat. Res. 51:383–396.

    PubMed  Google Scholar 

  7. Raposa, T. (1978).Mutat. Res. 57:241–251.

    PubMed  Google Scholar 

  8. Huff, V., DuFrain, R.J., and Littlefield, L.G. (1982).Mutat. Res. 94:349–357.

    PubMed  Google Scholar 

  9. Latt, S.A., and Loveday, K.S. (1978).Cytogenet. Cell Genet. 21:184–200.

    PubMed  Google Scholar 

  10. Morales-Ramírez, P., Vallarino-Kelly, T., and Rodríguez-Reyes, R. (1984).Environ. Mutagen. 6:529–537.

    PubMed  Google Scholar 

  11. Morales-Ramírez, P., Vallarino-Kelly, T., and Rodríguez-Reyes, R. (1988).Environ. Mol. Mutagen. 11:183–193.

    PubMed  Google Scholar 

  12. Morales-Ramírez, P., Rodríguez-Reyes, R., and Vallarino-Kelly, T. (1990).Mutat. Res. 232:77–88.

    PubMed  Google Scholar 

  13. He, S.-M., and Lambert, B. (1985).Mutat. Res. 158:201–208.

    Article  PubMed  Google Scholar 

  14. Nagasawa, H., Fornace, A.J., Ritter, M.A., and Little, J.B. (1982).Radiat. Res. 92:483–496.

    PubMed  Google Scholar 

  15. MacRae, W.D., MacKinnon, E.A., and Stich, H.F. (1979).Chromosoma 72:15–22.

    Article  PubMed  Google Scholar 

  16. Lambert, B., Sten, M., Soderhall, S., Ringborg, U., and Lewenshon, R. (1983).Mutat. Res. 111:171–184.

    PubMed  Google Scholar 

  17. Lambert, B., Sten, M., Hellgren, L., and Francesconi, D. (1984).Mutat. Res. 139:71–77.

    Article  PubMed  Google Scholar 

  18. Hedner, K., Mitelman, F., and Pero, R.W. (1984).Mutat. Res. 129:71–76.

    PubMed  Google Scholar 

  19. Linnainmaa, K., and Wolff, S. (1982).Environ. Mutagen. 4:239–248.

    PubMed  Google Scholar 

  20. Schwartz, J.L., Morgan, W.S., and Wolff, S. (1984).Environ. Mutagen. 6:203–210.

    PubMed  Google Scholar 

  21. Barka, T. (1965).Exp. Cell Res. 37:662–679.

    Article  PubMed  Google Scholar 

  22. Morales-Ramírez, P., Vallarino-Kelly, T., and Rodríguez-Reyes, R., (1984). InSister Chromatid Exchange, (eds.) Tice, R.R., and Hollaender, A. (Plenum Press, New York), pp 599–611.

    Google Scholar 

  23. Baserga, R. (1970).Fed. Proc. 29:1443–1446.

    PubMed  Google Scholar 

  24. Morales-Ramírez, P. (1980).Mutat. Res. 74:61–69.

    PubMed  Google Scholar 

  25. Morales-Ramírez, P., Mendiola-Cruz, M.T., Vallarino-Kelly, T., and Rodríguez-Reyes, R. (1994).Environ. Mol. Mutagen. 24:89–95.

    PubMed  Google Scholar 

  26. Goto, K., Akematsu, T., Shimazu, H., and Sugiyama, T. (1975).Chromosoma 53:223–230.

    Article  PubMed  Google Scholar 

  27. Perry, P., and Wolff, S. (1974).Nature 251:156–158.

    Article  PubMed  Google Scholar 

  28. Ishii, Y., and Bender, M.A. (1980).Mutat. Res. 79:19–32.

    Article  PubMed  Google Scholar 

  29. Raith, M., Cremer, T., Cremer, C., and Speit, G. (1984). InSister Chromatid Exchange, (eds.) Tice, R.R., and Hollaender, A., (Plenum Press, New York), pp 181–197.

    Google Scholar 

  30. Carrano, A.V., Thompson, L.H., Stetka, D.G., Minkley, J.L., Mazrimas, J.A., and Fong, S. (1979).Mutat. Res. 63:175–188.

    PubMed  Google Scholar 

  31. Cassel, D.M., and Latt, S.A. (1980).Exp. Cell Res. 128:15–22.

    Article  PubMed  Google Scholar 

  32. Ishii, Y. (1981).Mutat. Res. 91:51–55.

    Article  PubMed  Google Scholar 

  33. Kano, Y., and Fujiwara, Y. (1982).Mutat. Res. 103:327–332.

    Article  PubMed  Google Scholar 

  34. Kato, H. (1974).Nature 249:552–553.

    Article  PubMed  Google Scholar 

  35. Natarajan, A.T., van Zeeland, A.A., Vendergaal-Immerzeel, E.A.M., and Filon, A.R. (1980).Mutat. Res. 69:307–317.

    PubMed  Google Scholar 

  36. Daza, P., Escalza, P., Mateos, S., and Cortés, F. (1992).Mutat. Res. 270:177–183.

    PubMed  Google Scholar 

  37. Escalza, P., Daza, P., Piñero, J., and Cortés, F. (1992).Mutagenesis 7:137–140.

    PubMed  Google Scholar 

  38. Russo, A., Dorigo, E., Stocco, A., and Majone, F. (1993).Mutat. Res. 287:275–282.

    PubMed  Google Scholar 

  39. Morales-Ramírez, P., Vallarino-Kelly, T., and Rodríguez-Reyes, R. (1983).Environ. Mutagen. 2:149–155.

    Google Scholar 

  40. Abramovky, I., Vorsanger, G., and Kirschorn, K. (1978).Mutat. Res. 50:93–100.

    PubMed  Google Scholar 

  41. Perry, P., and Evans, H.J. (1975).Nature 258:121–125.

    Article  PubMed  Google Scholar 

  42. Anderson, H.C., Kihlman, B.A., and Palitti, F. (1981).Hereditas 94:41–44.

    Google Scholar 

  43. Renault, G., Gentil, S. and Chouroulinkov, I. (1982).Mutat. Res. 94:359–368.

    PubMed  Google Scholar 

  44. Fornace, A.J., Jr, Nagasawa, H., and Little, J.B. (1980).Mutat. Res. 70:323–336.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Departamento de Radiobiología, Instituto Nacional de Investigaciones Nucleares, Sierra Mojada No. 447 20 piso. Col. Lomas Barrilaco, C.P. 11010, México, D.F., México

    P. Morales-Ramírez, V. L. Cruz-Vallejo, T. Vallarino-Kelly & R. Rodríguez-Reyes

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  1. P. Morales-Ramírez
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  2. V. L. Cruz-Vallejo
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  3. T. Vallarino-Kelly
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  4. R. Rodríguez-Reyes
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Morales-Ramírez, P., Cruz-Vallejo, V.L., Vallarino-Kelly, T. et al. Persistence during G1 of gamma ray- or mitomycin C-induced lesions eliciting SCE in murine salivary gland cells in vivo. Somat Cell Mol Genet 21, 33–41 (1995). https://doi.org/10.1007/BF02255820

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  • DOI: https://doi.org/10.1007/BF02255820

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