Homology of Balbiani rings among chironomid species and localization of a new mobile element on the polytene chromosomes
The results ofin situ cross-hybridization of the cloned DNA fragments of BRa, BRb and BRc fromChironomus thummi to the polytene chromosomes of 14Chironomus species andCamptochironomus tentans are presented. BRs of the species studied were demonstrated to contain the homologus DNA sequences. The cloned fragment from the BRa ofC. thummi hybridized with the BRa ofC. piger and with a region on the arm G ofC. tentans andC. plumosus, the latter species had no extra BR. The clone λ 16 from the BRb ofC. thummi hybridized only with the developed BR on the arm G of all species studied. The sequence from the BRc ofC. thummi was located in the BRc ofC. piger and in the developed BR ofC. plumosus andC. nuditarsis, which were located at the most distal end of arm G. These clones can be used as markers of homologous BRs. The new mobile elements C6.10 fromC. thummi genome were localized on the polytene chromosomes of 10Chironomus species andCamptochironomus tentans. The species of the generaLipiniella, Cryptochironomus andGlyptotendipes did not contain the sequences homologous to ME C6.10.
KeywordsChironomidae Balbiani rings in situ hybridization mobile element
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- BEERMANN W., 1973. Directed changes in the pattern of Balbiani ring puffing inChironomus: effects of sugar treatment. Chromosoma (Berl.), 41: 297–326.Google Scholar
- BLINOV, A.G., Y.V. SOBANOV, E.K. GAIDAMAKOVA, S.S. BOGACHEV, N.N. KOLESNIKOV, M.A., FILIPPOVA and I.I. KIKNADZE. 1991. MEC: A transposable element fromChironomus thummi (Diptera). Mol. Gen. Genet., 229: 152–154.Google Scholar
- BOGACHEV S.S., A.G. BLINOV and N.N. KOLESNIKOV, 1990. A tissue-specific puff (Balbiani ring a) inChironomus thummi may contain a gene encoding a 67-kD a protein which exhibits non-tissue-specific expression. Gene, 96: 241–247.Google Scholar
- KIKNADZE, I.I., A.G. BLINOV and N.N. KOLESNIKOV, 1989. Molecular-cytological organisation of chironomid genome. In: V.K. Shumny, ed., Structural and functional organization of genome. Nauka, Novosibirsk, p. 4–58 (In Russian).Google Scholar
- LENDAHL, U. and L. WIESLANDER, 1985. Abrupt evolutionary change in the Balbiani ring gene family in two sibling species. J. Mol. Evol., 22: 63–68.Google Scholar
- WOBUS, U., H. BAUMLEIN, S.S. BOGACHEV, J.W. BORISEVICH, R. PANITZ and N.N. KOLESNIKOV, 1990. A new transposable element inChironomus thummi. Mol. Gen. Genet., 222: 311–316.Google Scholar