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Somatic Cell and Molecular Genetics

, Volume 20, Issue 3, pp 215–231 | Cite as

Tissue-specific extinguisher loci in the human genome: A screening study based on random marking and transfer of human chromosomes

  • Michael H. Shapero
  • Amelia A. Langston
  • R. E. K. Fournier
Article

Abstract

Expression of many liver-specific genes is extinguished when cultured hepatoma cells are fused with fibroblasts, but liver genes can be reexpressed in hybrid segregants that have lost fibroblast chromosomes. To map extinguisher loci involved in this process, hepatoma microcell hybrids retaining single fibroblast chromosomes have been employed. Two different,trans-dominant loci that affect liver gene expression have been defined in this way. To determine whether other monochromosomal extinction phenotypes could be observed, we inserted a selectable marker into many human chromosomal sites and transferred the marked human chromosomes into rat hepatoma recipient cells by microcell fusion. Nearly 200 microcell hybrid clones were isolated and screened for expression of liver-specific mRNAs. Most liver transcripts continued to be expressed. However, PEPCK mRNA was extinguished in 12 hybrid clones. Some of these hybrids contained humanTSE1, the previously characterized extinguisher locus on chromosome 17, but others contained a novel extinguishing function that mapped to human chromosome 14. The implications of these findings are discussed.

Keywords

Human Chromosome Selectable Marker Recipient Cell Hybrid Clone Liver Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Publishing Corporation 1994

Authors and Affiliations

  • Michael H. Shapero
    • 1
  • Amelia A. Langston
    • 1
  • R. E. K. Fournier
    • 1
  1. 1.Department of Molecular MedicineFred Hutchinson Cancer Research CenterSeattle

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